{"id":"W2098990266","doi":"10.1186/1475-2859-10-51","title":"Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield","year":2011,"lang":"en","type":"article","venue":"Microbial Cell Factories","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC","keywords":"Periplasmic space; Maltose-binding protein; Biochemistry; Fusion protein; Cytoplasm; Biology; Membrane protein; Chemistry; Biophysics; Cell biology; Membrane; Recombinant DNA; Escherichia coli; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000355777,0.000395012,0.0003959981,0.0001780595,0.0001928537,0.0005497149,0.0003628432,0.000300828,0.000458373],"category_scores_gemma":[0.0002662805,0.0001963181,0.0003747111,0.00045229,0.0001984491,0.0003246933,0.0003536068,0.0005884756,0.0004635858],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003265348,"about_ca_system_score_gemma":0.0002209752,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006952016,"about_ca_topic_score_gemma":0.0007463899,"domain_scores_codex":[0.9997476,0.0000534188,0.00004511598,0.00004229492,0.00007598695,0.0000355421],"domain_scores_gemma":[0.9998704,0.00002855598,0.00003473147,0.00002449131,0.00002430345,0.00001750584],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003759755,0.00002408502,0.0001644183,0.00003477851,0.000004273065,0.00003335093,0.00001870554,0.00006971431,0.9988984,0.00003649365,0.00002091134,0.0006572871],"study_design_scores_gemma":[0.000005653496,0.000097213,0.001199347,0.000005806306,0.000009437373,0.00015258,0.00002523848,0.0005732237,0.9965179,0.00001858344,0.001390019,0.000004935753],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9871538,0.001084379,0.01003142,0.0001282085,0.00002915745,0.00009106826,0.0007304739,0.00007873293,0.0006728026],"genre_scores_gemma":[0.9460318,0.002272393,0.04424987,0.00008432238,0.00001738966,0.0001277131,0.00367098,0.0001076667,0.003437805],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006952016,"threshold_uncertainty_score":0.002369165,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02767388157176044,"score_gpt":0.2356222946132232,"score_spread":0.2079484130414627,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}