{"id":"W2098990266","doi":"10.1186/1475-2859-10-51","title":"Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield","year":2011,"lang":"en","type":"article","venue":"Microbial Cell Factories","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC","keywords":"Periplasmic space; Maltose-binding protein; Biochemistry; Fusion protein; Cytoplasm; Biology; Membrane protein; Chemistry; Biophysics; Cell biology; Membrane; Recombinant DNA; Escherichia coli; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009629373,0.0001337839,0.0001630145,0.00004338702,0.00004849798,0.0000112348,0.0000860441,0.0001143517,0.00009620011],"category_scores_gemma":[0.00006356402,0.0001261091,0.00002437427,0.00007819474,0.0001176577,0.00001774814,0.00007019555,0.00007749959,0.000002142145],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002301657,"about_ca_system_score_gemma":0.00004177081,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004406608,"about_ca_topic_score_gemma":0.0001334979,"domain_scores_codex":[0.9990655,0.00006688464,0.0003061877,0.00032481,0.0001081439,0.0001284589],"domain_scores_gemma":[0.9994442,0.000008759046,0.000202635,0.0002482114,0.00004616303,0.00005004884],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0005918558,0.0002031922,0.002408486,0.00007386161,0.00001182484,5.692157e-7,0.000388401,0.000001408482,0.9961992,0.00003555647,0.000007342267,0.00007828436],"study_design_scores_gemma":[0.0002807853,0.0002497603,0.007478349,0.0001260158,0.000006031856,3.308909e-7,0.00005453195,4.612341e-7,0.989255,0.00001890657,0.00239685,0.0001329746],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9986991,0.00007690256,0.0002918881,0.000009407067,0.00002145243,0.0007218944,0.0001356865,0.000004283042,0.00003940086],"genre_scores_gemma":[0.9942895,0.00000963479,0.004916071,0.000007107576,0.00001730675,0.00004515263,0.0001283536,0.00001308559,0.0005737523],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.006944215,"threshold_uncertainty_score":0.5142581,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02767388157176044,"score_gpt":0.2356222946132232,"score_spread":0.2079484130414627,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}