{"id":"W2099229662","doi":"10.1038/ni.2050","title":"Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells","year":2011,"lang":"en","type":"article","venue":"Nature Immunology","topic":"Calcium signaling and nucleotide metabolism","field":"Biochemistry, Genetics and Molecular Biology","cited_by":304,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Alberta","funders":"National Institute of General Medical Sciences; National Institute of Allergy and Infectious Diseases; Canadian Institutes of Health Research","keywords":"Perforin; Granzyme; Endosome; Cell biology; Cytosol; Exocytosis; Endocytosis; Granzyme B; Chemistry; Cell membrane; Biology; Cell; Membrane; Intracellular; Biochemistry; Cytotoxic T cell","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003464763,0.0003077888,0.0004449382,0.0003183545,0.000284964,0.0009937666,0.0002606639,0.000738607,0.002281023],"category_scores_gemma":[0.0006208491,0.0002738801,0.0003942218,0.0002416181,0.0004225665,0.001263842,0.0009818401,0.001549398,0.001143457],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007650327,"about_ca_system_score_gemma":0.0002681884,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001643647,"about_ca_topic_score_gemma":0.0001916534,"domain_scores_codex":[0.9996346,0.00005750176,0.00002465925,0.00006446841,0.0001024775,0.0001162067],"domain_scores_gemma":[0.999745,0.00006339087,0.00004467964,0.00003926356,0.00003314133,0.00007455855],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004235349,0.00001441313,0.0001725924,0.00003646351,0.000007773212,0.0002332566,0.0000375967,0.00009479524,0.9968737,0.0007166696,0.00006217048,0.001327],"study_design_scores_gemma":[0.00009804948,0.0001641386,0.002269901,0.00001139637,0.00001312901,0.0006777995,0.00009080461,0.001521688,0.99265,0.0008698333,0.001623675,0.000009511038],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9870889,0.00161005,0.008657274,0.0004259701,0.00009287735,0.00002462474,0.0001774729,0.0001804256,0.001742412],"genre_scores_gemma":[0.9949239,0.0006472472,0.001646675,0.0001061844,0.00002759667,0.00002027074,0.000172596,0.00005293103,0.002402534],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002281023,"threshold_uncertainty_score":0.007630765,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007563031009880467,"score_gpt":0.2207792004243571,"score_spread":0.2132161694144766,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}