{"id":"W2099329633","doi":"10.1128/mbio.00189-10","title":"Membrane Topology Mapping of the O-Antigen Flippase (Wzx), Polymerase (Wzy), and Ligase (WaaL) from Pseudomonas aeruginosa PAO1 Reveals Novel Domain Architectures","year":2010,"lang":"en","type":"article","venue":"mBio","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":115,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; Stockholms Universitet; University of South Alabama","keywords":"Periplasmic space; Inner membrane; Biology; DNA ligase; Membrane protein; Transmembrane domain; Bacterial outer membrane; Biochemistry; Gene; Escherichia coli; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001856849,0.0002376201,0.0002837286,0.00006281855,0.0001268007,0.00002219346,0.0004202625,0.0005443868,0.00005259098],"category_scores_gemma":[0.0001039524,0.0001774746,0.0001213876,0.0001115173,0.0005455848,0.000001688221,0.0004305709,0.000305318,0.000002374032],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000003443377,"about_ca_system_score_gemma":0.00006082246,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004023047,"about_ca_topic_score_gemma":0.0005179376,"domain_scores_codex":[0.9986816,0.00006836744,0.0003287863,0.000480268,0.000108383,0.0003326112],"domain_scores_gemma":[0.9988997,0.00003082405,0.0001804522,0.0007452362,0.00004488275,0.00009890721],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007672553,0.00006927983,0.00388857,0.00001434935,0.0000688495,0.000003987572,0.00009124217,0.000001220991,0.9932522,0.0003226656,0.0001198259,0.002091123],"study_design_scores_gemma":[0.0007233611,0.0001162222,0.01350832,0.00001562911,0.00002207061,0.00006094875,0.00005190715,0.000005053512,0.9683641,0.0006627221,0.01625243,0.0002172774],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9959788,0.0005629941,0.0001800403,0.002021491,0.0005974685,0.0002499589,0.0002587595,0.00001617372,0.0001343121],"genre_scores_gemma":[0.9951213,0.0001365752,0.003466835,0.0006474592,0.0003852678,0.00001193819,0.00006544495,0.00002382926,0.000141378],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02488811,"threshold_uncertainty_score":0.7237206,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005877891279484859,"score_gpt":0.2071079636949698,"score_spread":0.201230072415485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}