{"id":"W2099329633","doi":"10.1128/mbio.00189-10","title":"Membrane Topology Mapping of the O-Antigen Flippase (Wzx), Polymerase (Wzy), and Ligase (WaaL) from Pseudomonas aeruginosa PAO1 Reveals Novel Domain Architectures","year":2010,"lang":"en","type":"article","venue":"mBio","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":115,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; Stockholms Universitet; University of South Alabama","keywords":"Periplasmic space; Inner membrane; Biology; DNA ligase; Membrane protein; Transmembrane domain; Bacterial outer membrane; Biochemistry; Gene; Escherichia coli; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00005865482,0.0002383457,0.0001265549,0.0001494491,0.0000929705,0.0002265037,0.0001185412,0.0001562785,0.0003225454],"category_scores_gemma":[0.0001505119,0.0001087029,0.0002246643,0.0001326795,0.0001412005,0.0001617331,0.0001675656,0.0002741819,0.0002180586],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002479259,"about_ca_system_score_gemma":0.000142285,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000762417,"about_ca_topic_score_gemma":0.0006015747,"domain_scores_codex":[0.9999579,0.000005669853,0.000004159249,0.00000914832,0.00001401475,0.000009046084],"domain_scores_gemma":[0.9999272,0.00001222532,0.00003236482,0.000005891891,0.00000896938,0.00001336655],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000440272,0.000005503739,0.0003454548,0.00001018969,0.000001948993,0.00003244963,0.0000129967,0.00007498569,0.999018,0.00003500483,0.000005510881,0.0004140122],"study_design_scores_gemma":[0.00001217433,0.0001432852,0.01941094,0.000007039461,0.00001683139,0.0004827002,0.0001014873,0.003911029,0.9748198,0.00008534658,0.001000956,0.000008384437],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9981857,0.00008928524,0.001456633,0.00002115035,0.00000182,0.000005401314,0.0001278917,0.00001220096,0.00009994104],"genre_scores_gemma":[0.9962807,0.000147767,0.002228348,0.0000156981,0.000002087649,0.00001294489,0.0008229889,0.00001258709,0.0004768884],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000762417,"threshold_uncertainty_score":0.001798809,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005877891279484859,"score_gpt":0.2071079636949698,"score_spread":0.201230072415485,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}