{"id":"W2099839597","doi":"10.1021/bi051292p","title":"Purification and Characterization of a Recombinant G-Protein-Coupled Receptor, <i>Saccharomyces cerevisiae</i> Ste2p, Transiently Expressed in HEK293 EBNA1 Cells","year":2005,"lang":"en","type":"article","venue":"Biochemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"Plant Biotechnology Institute; Biotechnology Research Institute; University of Saskatchewan","funders":"University of Saskatchewan","keywords":"Fusion protein; G protein-coupled receptor; Green fluorescent protein; Saccharomyces cerevisiae; Chemistry; Ligand (biochemistry); Biochemistry; Receptor; HEK 293 cells; Affinity chromatography; Heterologous expression; Ligand binding assay; Endosome; Recombinant DNA; Biophysics; Biology; Yeast","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002148223,0.0001892872,0.0002212028,0.00003894946,0.00003999644,0.00002557633,0.0001590106,0.0002330868,0.00009395329],"category_scores_gemma":[0.00001923156,0.0001948202,0.00006647254,0.0001019866,0.00004519575,0.00001206727,0.00003705934,0.00008512916,0.00000321618],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001932883,"about_ca_system_score_gemma":0.00005146482,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000103639,"about_ca_topic_score_gemma":0.000001727705,"domain_scores_codex":[0.9987834,0.00003818018,0.0003985101,0.0004358222,0.0001352232,0.0002088574],"domain_scores_gemma":[0.999357,0.000006119707,0.0001965526,0.0002927339,0.00007141521,0.00007615772],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000896406,0.0001054521,0.00007116471,0.0001263336,0.00001295609,3.766825e-7,0.00007658439,0.000004609555,0.996502,0.00001219809,0.0000521864,0.002946454],"study_design_scores_gemma":[0.0006873751,0.00007889943,0.000102709,0.0001104274,0.000007868173,0.000002232419,0.00007056328,0.0002032091,0.9946266,0.000009669843,0.003887298,0.0002131103],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9980928,0.000568941,0.0006818292,0.0001561373,0.00008583606,0.0003002192,0.00003217669,0.00001152258,0.00007056908],"genre_scores_gemma":[0.9967164,0.000654769,0.001538853,0.00004068973,0.0001426046,0.00005597537,0.0003160719,0.00002486402,0.0005097586],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003835111,"threshold_uncertainty_score":0.7944535,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005761556083602085,"score_gpt":0.2003150357492801,"score_spread":0.194553479665678,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}