{"id":"W2099839597","doi":"10.1021/bi051292p","title":"Purification and Characterization of a Recombinant G-Protein-Coupled Receptor, <i>Saccharomyces cerevisiae</i> Ste2p, Transiently Expressed in HEK293 EBNA1 Cells","year":2005,"lang":"en","type":"article","venue":"Biochemistry","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"Plant Biotechnology Institute; Biotechnology Research Institute; University of Saskatchewan","funders":"University of Saskatchewan","keywords":"Fusion protein; G protein-coupled receptor; Green fluorescent protein; Saccharomyces cerevisiae; Chemistry; Ligand (biochemistry); Biochemistry; Receptor; HEK 293 cells; Affinity chromatography; Heterologous expression; Ligand binding assay; Endosome; Recombinant DNA; Biophysics; Biology; Yeast","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002246546,0.0004486012,0.0004493669,0.0001822093,0.0001548296,0.0004862885,0.0003515304,0.0002928698,0.0007514811],"category_scores_gemma":[0.0002314689,0.0001751666,0.0003567395,0.0003637818,0.0001248595,0.0002091404,0.0002137065,0.0005420566,0.001214327],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003182191,"about_ca_system_score_gemma":0.0001921326,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001099138,"about_ca_topic_score_gemma":0.001001019,"domain_scores_codex":[0.9998733,0.00001810929,0.00001690123,0.00002676558,0.00004601476,0.00001894421],"domain_scores_gemma":[0.9998827,0.000018247,0.00001709169,0.00001734846,0.00004036309,0.00002422475],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002198798,0.00001305558,0.0001202693,0.00003430259,0.00000410073,0.0000477413,0.00001343214,0.00006109659,0.999065,0.00004530698,0.00004954647,0.0005241244],"study_design_scores_gemma":[0.00001041318,0.00009525323,0.002295223,0.000007451437,0.00001473659,0.000380692,0.00004509075,0.001382717,0.9900823,0.00003610808,0.005642976,0.000006994132],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9596194,0.00163744,0.02906863,0.0003976923,0.0000593842,0.0001260532,0.006229673,0.0004351697,0.002426532],"genre_scores_gemma":[0.9330157,0.002899591,0.03427477,0.0001989133,0.00002766714,0.0001649814,0.02006493,0.0003022068,0.009051147],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001099138,"threshold_uncertainty_score":0.002514005,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005761556083602085,"score_gpt":0.2003150357492801,"score_spread":0.194553479665678,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}