{"id":"W2099964206","doi":"10.1186/1472-6750-6-49","title":"Multiplexed expression and screening for recombinant protein production in mammalian cells","year":2006,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Research Council Canada; University of Oxford; Wellcome Trust","keywords":"Biology; HEK 293 cells; Recombinant DNA; Western blot; Transfection; Molecular biology; Blot; FLAG-tag; Expression vector; Cell culture; Gene; Biochemistry; Fusion protein; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.004923209,0.001981539,0.002448074,0.002368949,0.0004931737,0.002077564,0.001485771,0.001746957,0.003046094],"category_scores_gemma":[0.003398984,0.00115736,0.001552102,0.001882885,0.0008765361,0.001000243,0.001576802,0.002522905,0.004228448],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0007401747,"about_ca_system_score_gemma":0.0005098476,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003507673,"about_ca_topic_score_gemma":0.0005053005,"domain_scores_codex":[0.992465,0.001991092,0.0008963526,0.001579233,0.002632217,0.0004361803],"domain_scores_gemma":[0.9977207,0.0008345872,0.0003478802,0.0004478625,0.0005001736,0.0001488563],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007184522,0.00008198036,0.0001947558,0.0001887543,0.00002929169,0.00008862271,0.00005051415,0.0002485158,0.9939967,0.0002080213,0.0002164933,0.004624486],"study_design_scores_gemma":[0.00002310663,0.0003057501,0.0007852694,0.00002650872,0.00006518747,0.0003419895,0.00002714088,0.003613488,0.9890677,0.0001684495,0.005544215,0.00003106131],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1194587,0.002755003,0.8647968,0.0004537721,0.000371594,0.003276723,0.003002742,0.003282706,0.00260191],"genre_scores_gemma":[0.1426605,0.003870903,0.8329777,0.0003347948,0.0001472747,0.004560151,0.007725421,0.0006051788,0.007118125],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.004923209,"threshold_uncertainty_score":0.02603674,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01232814378323729,"score_gpt":0.2418296214402641,"score_spread":0.2295014776570268,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}