{"id":"W2099964206","doi":"10.1186/1472-6750-6-49","title":"Multiplexed expression and screening for recombinant protein production in mammalian cells","year":2006,"lang":"en","type":"article","venue":"BMC Biotechnology","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"National Research Council Canada; University of Oxford; Wellcome Trust","keywords":"Biology; HEK 293 cells; Recombinant DNA; Western blot; Transfection; Molecular biology; Blot; FLAG-tag; Expression vector; Cell culture; Gene; Biochemistry; Fusion protein; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000115318,0.0001369021,0.0001244253,0.0001082165,0.00009894878,0.00001405834,0.0000923424,0.0003912946,0.000003873169],"category_scores_gemma":[0.00008148831,0.0001267903,0.00004988276,0.00009150336,0.00008761037,0.000004993633,0.0001007867,0.00008866636,0.000001473422],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000009747266,"about_ca_system_score_gemma":0.00001980368,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001037471,"about_ca_topic_score_gemma":0.0002297282,"domain_scores_codex":[0.9990109,0.00003937802,0.000199272,0.0004600057,0.00004605865,0.0002443742],"domain_scores_gemma":[0.9995893,0.000004954841,0.00008570786,0.0002603974,0.00003012432,0.00002953999],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001664655,0.00008852254,0.002110988,0.00004516661,0.000002523115,8.430443e-7,0.000003190762,0.0001153819,0.9931093,0.00010786,0.001798074,0.002451703],"study_design_scores_gemma":[0.0008594601,0.0002863869,0.001821805,0.00004663476,0.000003802932,0.000007744386,0.00003394703,0.0001048045,0.9863381,0.00073884,0.009589246,0.0001692499],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9897391,0.0005533061,0.008297863,0.0002962813,0.0002294219,0.0007948895,0.00001011343,0.00004988295,0.00002917461],"genre_scores_gemma":[0.9681532,0.00003323192,0.03064012,0.00002568196,0.0001444061,0.000281834,0.00005329531,0.00002160909,0.0006465997],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02234226,"threshold_uncertainty_score":0.5170358,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01232814378323729,"score_gpt":0.2418296214402641,"score_spread":0.2295014776570268,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}