{"id":"W2100195727","doi":"10.1177/1087057109357790","title":"A Fluorescent Protein-Based Biological Screen of Proteinase Activity","year":2010,"lang":"en","type":"article","venue":"SLAS DISCOVERY","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Förster resonance energy transfer; Linker; Cleavage (geology); Fluorescence; Cysteine; Plate reader; Chemistry; Green fluorescent protein; Biochemistry; Escherichia coli; Molecular biology; Biophysics; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002131104,0.0001097201,0.0002439081,0.0001108844,0.00006869734,0.00001685399,0.00008523399,0.0001581281,0.0002333421],"category_scores_gemma":[0.000304891,0.0000745026,0.000130548,0.0001903165,0.0003961236,0.0001232749,0.00006257555,0.0005435731,0.00003816248],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002324825,"about_ca_system_score_gemma":0.0002823559,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0003285792,"about_ca_topic_score_gemma":0.00004181949,"domain_scores_codex":[0.9991731,0.000062297,0.0001441808,0.0002106433,0.000161478,0.0002482673],"domain_scores_gemma":[0.9993789,0.00003067453,0.00005725919,0.0003749123,0.00008872572,0.00006958582],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007981015,0.0007218986,0.02177733,0.00004500392,0.00004161333,0.00001551131,0.00000924274,5.582046e-7,0.9693058,0.001220154,0.00007575299,0.005989067],"study_design_scores_gemma":[0.001243861,0.001629811,0.1508445,0.0000729272,0.00001578987,0.00001222725,0.000018399,0.0001242218,0.844533,0.0001995989,0.001204025,0.0001016303],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9964987,0.00004189802,0.0004855665,0.0006629603,0.00007514859,0.0010061,0.00002506114,0.00003795262,0.001166623],"genre_scores_gemma":[0.9986949,0.000004922763,0.000466352,0.00004215408,0.00007182195,0.0001180433,0.00001524159,0.00001047484,0.0005761098],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1290672,"threshold_uncertainty_score":0.3038128,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0284261334427979,"score_gpt":0.3112579493739066,"score_spread":0.2828318159311087,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}