{"id":"W2100195727","doi":"10.1177/1087057109357790","title":"A Fluorescent Protein-Based Biological Screen of Proteinase Activity","year":2010,"lang":"en","type":"article","venue":"SLAS DISCOVERY","topic":"Viral Infections and Immunology Research","field":"Medicine","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Förster resonance energy transfer; Linker; Cleavage (geology); Fluorescence; Cysteine; Plate reader; Chemistry; Green fluorescent protein; Biochemistry; Escherichia coli; Molecular biology; Biophysics; Biology; Enzyme; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00072541,0.001005016,0.001289158,0.0008876925,0.0003858075,0.000581214,0.0006423741,0.001203404,0.001229292],"category_scores_gemma":[0.0004651986,0.0003802568,0.0006899353,0.000768402,0.0003208154,0.000346498,0.0004993408,0.00113866,0.0008749628],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006916664,"about_ca_system_score_gemma":0.0004244063,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005881711,"about_ca_topic_score_gemma":0.001172792,"domain_scores_codex":[0.9988952,0.0001907593,0.00008036626,0.0002066163,0.0004568508,0.000170124],"domain_scores_gemma":[0.9996244,0.0001094291,0.00006795794,0.00004677385,0.00008970046,0.00006172335],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002414736,0.00003669368,0.00006548566,0.00003680446,0.000005435419,0.00004269405,0.000007452328,0.00005806218,0.9984962,0.00004697097,0.0000624377,0.001117584],"study_design_scores_gemma":[0.00001172072,0.0002994375,0.0009058152,0.00000396425,0.00001540802,0.0002648162,0.00000967938,0.0008431784,0.9951525,0.00003263204,0.002452308,0.000008541803],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7681546,0.005042739,0.2084082,0.0008194476,0.0001795535,0.001422672,0.006466375,0.001933028,0.007573276],"genre_scores_gemma":[0.8358324,0.00408737,0.1369624,0.0004145369,0.00004265501,0.001408134,0.0067921,0.0001962619,0.01426429],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001289158,"threshold_uncertainty_score":0.005018413,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0284261334427979,"score_gpt":0.3112579493739066,"score_spread":0.2828318159311087,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}