{"id":"W2100284529","doi":"10.1007/s10930-009-9216-x","title":"Studies of Glu-416 Variants of β-Galactosidase (E. coli) Show that the Active Site Mg2+ is Not Important for Structure and Indicate that the Main Role of Mg2+ is to Mediate Optimization of Active Site Chemistry","year":2009,"lang":"en","type":"article","venue":"The Protein Journal","topic":"Enzyme Catalysis and Immobilization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":24,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of Calgary","funders":"Directorate for Biological Sciences; University of Calgary","keywords":"Active site; Chemistry; Glutamine; Stereochemistry; Substrate (aquarium); Enzyme; Valine; Biochemistry; Amino acid; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002239709,0.0007447231,0.000389136,0.0001788041,0.0002620421,0.0005000672,0.0006070649,0.0003962787,0.001316718],"category_scores_gemma":[0.0003466182,0.0001924489,0.0004439881,0.000423135,0.0003820269,0.000254509,0.000275885,0.0008719699,0.0006387907],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004382755,"about_ca_system_score_gemma":0.0001638967,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00187121,"about_ca_topic_score_gemma":0.001302071,"domain_scores_codex":[0.9997266,0.00006364242,0.00003422006,0.00004438282,0.00008340863,0.00004778682],"domain_scores_gemma":[0.9997756,0.00005897629,0.00005355966,0.00003475104,0.00002895478,0.00004816461],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000490137,0.00009024715,0.0005788837,0.00006438691,0.00003455272,0.0002108662,0.00006320409,0.0002234559,0.9963943,0.0003170813,0.00006045315,0.001472343],"study_design_scores_gemma":[0.00001038797,0.000168677,0.001470745,0.000003474398,0.00001892351,0.0004026502,0.00004461916,0.0005812945,0.995526,0.00006520996,0.001699768,0.000008213504],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9940439,0.0006225753,0.003312329,0.00009330823,0.00003232608,0.00001502001,0.000420578,0.00004004294,0.001419845],"genre_scores_gemma":[0.9934791,0.0003370802,0.001998748,0.00004499995,0.000006523598,0.000009318281,0.001602282,0.0001063708,0.002415491],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00187121,"threshold_uncertainty_score":0.004404902,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0122646889015418,"score_gpt":0.2554997364181713,"score_spread":0.2432350475166295,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}