{"id":"W2101763866","doi":"10.1093/protein/gzt052","title":"Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry","year":2013,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; University of Alberta","keywords":"mCherry; Fluorescence; Fluorescent protein; Green fluorescent protein; Biophysics; Calmodulin; Calcium; Directed evolution; Phototoxicity; Fusion protein; Chemistry; Biology; Biochemistry; Computational biology; Recombinant DNA; Mutant; Optics; Gene; In vitro; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001286581,0.0001776636,0.0001067589,0.0001327406,0.00007652671,0.00003751806,0.00005269201,0.0001688253,0.00000567594],"category_scores_gemma":[0.00007535259,0.0001760096,0.00002341671,0.0001480745,0.00003414787,0.00001056948,0.00002023838,0.0001498308,0.000001490348],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003649878,"about_ca_system_score_gemma":0.00002572073,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000159564,"about_ca_topic_score_gemma":2.344176e-7,"domain_scores_codex":[0.9991878,0.00004654254,0.0001285431,0.0003305239,0.00009086624,0.0002157172],"domain_scores_gemma":[0.9997155,0.000005828485,0.00004157989,0.0001075047,0.00004327666,0.00008633107],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004125146,0.00002966732,0.0001799546,0.00004653485,0.000009483441,7.583085e-7,0.00001248268,0.001659072,0.9960753,0.00001133732,0.0000474448,0.001886743],"study_design_scores_gemma":[0.000258599,0.0004685606,0.001470671,0.00005969408,0.000005400368,0.000006442729,0.000002874937,0.06085777,0.9362434,0.00002790374,0.0004030464,0.0001957021],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.2611979,0.0001651777,0.7371709,0.00007286557,0.00001738407,0.001260956,8.158129e-7,0.0001078049,0.000006194776],"genre_scores_gemma":[0.9168602,0.00002529665,0.08245394,0.00003806363,0.00004797424,0.0004967092,0.00001368765,0.0000353269,0.00002883826],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.6556622,"threshold_uncertainty_score":0.7177464,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006432532036446647,"score_gpt":0.2154521213689298,"score_spread":0.2090195893324831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}