{"id":"W2101763866","doi":"10.1093/protein/gzt052","title":"Circular permutated red fluorescent proteins and calcium ion indicators based on mCherry","year":2013,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":26,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; University of Alberta","keywords":"mCherry; Fluorescence; Fluorescent protein; Green fluorescent protein; Biophysics; Calmodulin; Calcium; Directed evolution; Phototoxicity; Fusion protein; Chemistry; Biology; Biochemistry; Computational biology; Recombinant DNA; Mutant; Optics; Gene; In vitro; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003268696,0.0004173573,0.0003795893,0.0002451946,0.0001506876,0.000434395,0.0004500102,0.0004131778,0.0009626477],"category_scores_gemma":[0.000408131,0.0002620599,0.0003129367,0.0004497598,0.0004986383,0.0003276346,0.0004019325,0.0007914282,0.0005501657],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004356655,"about_ca_system_score_gemma":0.000248423,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000405896,"about_ca_topic_score_gemma":0.0007183936,"domain_scores_codex":[0.9997978,0.00003541993,0.00001743369,0.00005379644,0.000062966,0.00003269363],"domain_scores_gemma":[0.9997726,0.00004974359,0.00008253792,0.00002549552,0.00002895669,0.00004057449],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006580813,0.00001480607,0.0001375582,0.00003793107,0.000004551869,0.00003714081,0.00001520978,0.0002415809,0.9971145,0.000397009,0.00002667919,0.001907139],"study_design_scores_gemma":[0.00001359526,0.0002774532,0.0009065869,0.000005159587,0.00001229807,0.0002158583,0.00001176482,0.00132849,0.9937786,0.00008370018,0.003356105,0.00001024932],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9436005,0.00152935,0.05125175,0.0001838895,0.00004500435,0.000208204,0.0003368397,0.0002713626,0.002573072],"genre_scores_gemma":[0.8913269,0.001809107,0.09958076,0.000157973,0.00001570206,0.0001853941,0.001093856,0.0001239456,0.005706371],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009626477,"threshold_uncertainty_score":0.003220379,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006432532036446647,"score_gpt":0.2154521213689298,"score_spread":0.2090195893324831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}