{"id":"W2101906343","doi":"10.2144/000112266","title":"Whole Genome Amplification of Sodium Bisulfite-Treated DNA Allows the Accurate Estimate of Methylated Cytosine Density in Limited DNA Resources","year":2006,"lang":"en","type":"article","venue":"BioTechniques","topic":"Epigenetics and DNA Methylation","field":"Biochemistry, Genetics and Molecular Biology","cited_by":34,"is_retracted":false,"has_abstract":true,"ca_institutions":"Centre for Addiction and Mental Health","funders":"Stanley Foundation; National Institute of Mental Health; National Alliance for Research on Schizophrenia and Depression","keywords":"Sodium bisulfite; Bisulfite; DNA methylation; Methylated DNA immunoprecipitation; Bisulfite sequencing; DNA; Molecular biology; Primer (cosmetics); genomic DNA; Illumina Methylation Assay; Biology; Methylation; Chemistry; Genetics; Gene; Gene expression","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004804884,0.000654414,0.0005800782,0.0006746273,0.0002788994,0.000425195,0.00038569,0.0005241975,0.001248875],"category_scores_gemma":[0.0008100796,0.0003802077,0.000399093,0.0005684302,0.0003697045,0.0004005192,0.0003645004,0.0006286069,0.0009536993],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002644839,"about_ca_system_score_gemma":0.0004015388,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00078936,"about_ca_topic_score_gemma":0.002213098,"domain_scores_codex":[0.9996241,0.00008513378,0.00002105122,0.0001309169,0.0001142193,0.00002444284],"domain_scores_gemma":[0.9995897,0.0001419571,0.00009146961,0.00007634261,0.00007441259,0.00002607849],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00002608494,0.000008372586,0.0005975019,0.00006627137,0.00001470183,0.00001419557,0.0000219953,0.0001550231,0.9925419,0.0001330784,0.00003748423,0.006383433],"study_design_scores_gemma":[0.000004026577,0.0001401823,0.01052786,0.00001332048,0.00004475306,0.0001438614,0.00002525008,0.002239214,0.9796472,0.0003600247,0.006842121,0.00001230437],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6040404,0.00750752,0.3797901,0.00023414,0.00008328223,0.0001696048,0.002882504,0.001425226,0.0038672],"genre_scores_gemma":[0.731057,0.006681124,0.2466682,0.0001750665,0.00002938579,0.000259649,0.008157719,0.0002740027,0.006697717],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001248875,"threshold_uncertainty_score":0.004177868,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.0129409217788598,"score_gpt":0.265672923215465,"score_spread":0.2527320014366052,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}