{"id":"W2102220938","doi":"10.1128/aem.02220-07","title":"Improvement of the Thermostability and Activity of a Pectate Lyase by Single Amino Acid Substitutions, Using a Strategy Based on Melting-Temperature-Guided Sequence Alignment","year":2007,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Enzyme Production and Characterization","field":"Biochemistry, Genetics and Molecular Biology","cited_by":67,"is_retracted":false,"has_abstract":true,"ca_institutions":"National Research Council Canada; Biotechnology Research Institute","funders":"Natural Resources Canada","keywords":"Thermostability; Pectate lyase; Thermophile; Directed evolution; Mutagenesis; Biology; Amino acid; Biochemistry; Mesophile; Enzyme; Peptide sequence; Mutation; Gene; Genetics; Bacteria; Mutant","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003534073,0.0005504235,0.0004494729,0.0002510283,0.0001982456,0.0003888258,0.0002156332,0.0004149846,0.0004900528],"category_scores_gemma":[0.0004217472,0.0001511331,0.0003082064,0.0002979431,0.0002496016,0.0002076988,0.0003084327,0.0004984965,0.0003669028],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001665695,"about_ca_system_score_gemma":0.0002089132,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002437099,"about_ca_topic_score_gemma":0.000325416,"domain_scores_codex":[0.9997428,0.00006152778,0.00003789864,0.00005988404,0.00007036477,0.00002756362],"domain_scores_gemma":[0.9998162,0.00004848138,0.00006480099,0.0000306871,0.00001857385,0.00002114459],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000348091,0.00001847511,0.0001990929,0.00001371331,0.000005902534,0.00006475001,0.00001040581,0.0001262024,0.9973466,0.00003575907,0.000006172903,0.002138037],"study_design_scores_gemma":[0.000005880348,0.0002339443,0.00121008,0.00000213254,0.00001656979,0.001148246,0.00001277434,0.00150562,0.9950099,0.00002502336,0.0008239765,0.00000574761],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9832486,0.0005172574,0.01522849,0.0001032651,0.00001511772,0.00007589452,0.0001116545,0.000135644,0.0005639709],"genre_scores_gemma":[0.9804662,0.0004286328,0.01820163,0.00003342979,0.000007493297,0.00002933098,0.0001875496,0.00004746499,0.0005983234],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005504235,"threshold_uncertainty_score":0.001869023,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01100165582583951,"score_gpt":0.2162700569711652,"score_spread":0.2052684011453257,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}