{"id":"W2103412476","doi":"10.2174/092986611794927901","title":"Site-Directed Mutagenesis to Identify Key Residues in Structure-Function Relationship of Winged Bean Chymotrypsin-Trypsin Inhibitor and 3-D Structure Prediction","year":2011,"lang":"en","type":"article","venue":"Protein and Peptide Letters","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":2,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"Petroleum Technology Research Centre; Council of Scientific and Industrial Research, India","keywords":"Trypsin; Chymotrypsin; Proteases; Biochemistry; Serine; Site-directed mutagenesis; Serine protease; Protease; Mutant; Mutagenesis; Trypsin inhibitor; Enzyme; Chemistry; Kunitz STI protease inhibitor; Biology; Molecular biology; Gene","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002989307,0.000444578,0.0003107565,0.0002499686,0.0002024454,0.0001855358,0.0003656195,0.0002871964,0.001361726],"category_scores_gemma":[0.000330422,0.0002498278,0.0004251731,0.0002874786,0.0001340661,0.0001237216,0.0001734957,0.0005573382,0.0003652636],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003761988,"about_ca_system_score_gemma":0.0004281156,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001615845,"about_ca_topic_score_gemma":0.002330354,"domain_scores_codex":[0.999869,0.00003622656,0.00001649659,0.00002302125,0.00003930297,0.00001592032],"domain_scores_gemma":[0.999851,0.00004842069,0.00003363166,0.00001717854,0.00002565543,0.00002416563],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002509388,0.0001594913,0.001133086,0.00009543564,0.00002573733,0.0003885293,0.00005333326,0.004275596,0.987753,0.0006881328,0.0002015886,0.004975029],"study_design_scores_gemma":[0.0001213493,0.0005181928,0.009568643,0.00002094836,0.0001081128,0.000937922,0.00008916033,0.07252871,0.9075603,0.0003845522,0.008131427,0.00003069544],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9441411,0.0005637457,0.05232963,0.0001417621,0.00005591962,0.0002729369,0.0007257343,0.0002796088,0.001489511],"genre_scores_gemma":[0.9333497,0.0004442056,0.06340089,0.00006327662,0.000007086744,0.0001222238,0.001185985,0.00006754352,0.001359093],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001615845,"threshold_uncertainty_score":0.004555404,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01059952263908898,"score_gpt":0.2225936409033757,"score_spread":0.2119941182642868,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}