{"id":"W2104463361","doi":"10.1002/bit.22698","title":"High‐level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene‐switch","year":2010,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institut National de la Recherche Scientifique; McGill University; Montreal Neurological Institute and Hospital; Université de Montréal; Université Laval; National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Chinese hamster ovary cell; Internal ribosome entry site; Green fluorescent protein; Recombinant DNA; Molecular biology; Multiplicity of infection; Biology; Viral vector; Transfection; Transactivation; Cell culture; Transduction (biophysics); Genetic enhancement; Protein biosynthesis; Reporter gene; Gene expression; Gene; Messenger RNA; Translation (biology); Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001696415,0.0001432577,0.0001286803,0.0000862856,0.0001152219,0.00002250981,0.00008697428,0.0003108532,0.000002346433],"category_scores_gemma":[0.00004285721,0.0001045473,0.00003073838,0.0001070823,0.0002101529,0.000007451723,0.0001304038,0.0002425658,7.498241e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000006232447,"about_ca_system_score_gemma":0.00001470714,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00008960928,"about_ca_topic_score_gemma":0.00005514689,"domain_scores_codex":[0.9992794,0.00002160594,0.0001459509,0.0003057272,0.00004155637,0.0002057549],"domain_scores_gemma":[0.9996825,0.000003410347,0.00004881889,0.0002174068,0.00001605058,0.00003176822],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005517832,0.00002283807,0.000557382,0.00002090029,0.00001757867,0.000001794763,0.00001323636,0.0001188597,0.9972051,0.000407995,0.00002066542,0.001558442],"study_design_scores_gemma":[0.0006298156,0.00006600057,0.0008853917,0.0000235898,0.0000108088,0.00003921965,0.00001883676,0.000687392,0.9965268,0.0002282726,0.0007326251,0.0001512222],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9973419,0.0004862854,0.0004856593,0.0003886114,0.0008942967,0.0003604158,0.000005031262,0.0000340115,0.00000375073],"genre_scores_gemma":[0.9983847,0.0003094759,0.001025991,0.00001890384,0.0001368313,0.00004640485,0.000005659112,0.00001455576,0.00005755234],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00140722,"threshold_uncertainty_score":0.4263315,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007325940197718539,"score_gpt":0.2097888369277328,"score_spread":0.2024628967300142,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}