{"id":"W2104463361","doi":"10.1002/bit.22698","title":"High‐level recombinant protein production in CHO cells using lentiviral vectors and the cumate gene‐switch","year":2010,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Viral Infectious Diseases and Gene Expression in Insects","field":"Biochemistry, Genetics and Molecular Biology","cited_by":61,"is_retracted":false,"has_abstract":true,"ca_institutions":"Institut National de la Recherche Scientifique; McGill University; Montreal Neurological Institute and Hospital; Université de Montréal; Université Laval; National Research Council Canada; Biotechnology Research Institute","funders":"","keywords":"Chinese hamster ovary cell; Internal ribosome entry site; Green fluorescent protein; Recombinant DNA; Molecular biology; Multiplicity of infection; Biology; Viral vector; Transfection; Transactivation; Cell culture; Transduction (biophysics); Genetic enhancement; Protein biosynthesis; Reporter gene; Gene expression; Gene; Messenger RNA; Translation (biology); Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000675961,0.0006421819,0.0005982544,0.0004452732,0.0002783182,0.0008212829,0.0005862847,0.0004642286,0.001031418],"category_scores_gemma":[0.0002954522,0.0002796559,0.0005449952,0.0004655813,0.000394648,0.0004552354,0.0005387859,0.001139244,0.001305402],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005356513,"about_ca_system_score_gemma":0.0004041858,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007362345,"about_ca_topic_score_gemma":0.0007187407,"domain_scores_codex":[0.9996555,0.00005674253,0.00005684132,0.00007457506,0.0001137548,0.00004256471],"domain_scores_gemma":[0.9998518,0.00002956999,0.00003145975,0.00003856029,0.00002852557,0.00002008407],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000284275,0.00001334038,0.00005990895,0.00005042689,0.000008168681,0.00003936846,0.00002099721,0.00006389242,0.9980615,0.000215818,0.00006851122,0.001369614],"study_design_scores_gemma":[0.00002419336,0.0001451325,0.0004610626,0.00001039496,0.0000315377,0.0001825494,0.00001320064,0.000908223,0.9882635,0.00010223,0.009847949,0.00001000162],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.7088159,0.005188499,0.2676952,0.0004853509,0.0003155911,0.00117826,0.005416389,0.002434306,0.008470557],"genre_scores_gemma":[0.7614125,0.006205826,0.2039957,0.0002356678,0.0001193163,0.001132051,0.01163725,0.0006977241,0.01456393],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001031418,"threshold_uncertainty_score":0.003886461,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007325940197718539,"score_gpt":0.2097888369277328,"score_spread":0.2024628967300142,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}