{"id":"W2105073997","doi":"10.1016/j.pep.2004.07.016","title":"General co-expression vectors for the overexpression of heterodimeric protein complexes in Escherichia coli","year":2004,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":36,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Allen Foundation; National Institute of Allergy and Infectious Diseases; University of Texas Southwestern Medical Center; Ryerson University; University of Michigan; Rita Allen Foundation; National Institutes of Health; Pew Charitable Trusts","keywords":"Escherichia coli; Plasmid; Cloning (programming); Expression vector; Cloning vector; Origin of replication; Biology; Recombinant DNA; Vector (molecular biology); Kanamycin; Computational biology; Multiple cloning site; Molecular biology; Gene; Genetics; Computer science","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0009093415,0.001967951,0.001759269,0.001561088,0.0009969847,0.001360399,0.001791673,0.001270865,0.006870448],"category_scores_gemma":[0.001096191,0.001420202,0.00095973,0.002288779,0.0004778944,0.0008466254,0.0008634508,0.003043462,0.01189325],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009806242,"about_ca_system_score_gemma":0.0007213277,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001443726,"about_ca_topic_score_gemma":0.002177965,"domain_scores_codex":[0.9987858,0.0001972758,0.0001968723,0.0002327166,0.0004099051,0.000177494],"domain_scores_gemma":[0.9992161,0.0001937043,0.00007682354,0.0002046133,0.0001521955,0.0001565632],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000403881,0.0002837865,0.0002766385,0.0005745869,0.0000496372,0.0001358137,0.0001117697,0.0002973376,0.9830737,0.001882432,0.004503101,0.008407342],"study_design_scores_gemma":[0.0001196759,0.0001802403,0.001742414,0.00006206951,0.00009205537,0.000639611,0.0000574553,0.002540236,0.9353529,0.0004655063,0.05869713,0.00005056756],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.350247,0.01093482,0.535422,0.002344257,0.002162044,0.006154129,0.0575202,0.01093197,0.02428368],"genre_scores_gemma":[0.3581702,0.01158033,0.4217721,0.0008164534,0.0003440373,0.006449233,0.1227215,0.003460804,0.07468536],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.006870448,"threshold_uncertainty_score":0.02298397,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01400382809932123,"score_gpt":0.2574828108865345,"score_spread":0.2434789827872132,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}