{"id":"W2106203631","doi":"10.1002/bit.20806","title":"Affinity‐tagged green fluorescent protein (GFP) extraction from a clarified <i>E. coli</i> cell lysate using a two‐phase aqueous micellar system","year":2006,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Surfactants and Colloidal Systems","field":"Chemistry","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"National Institute of General Medical Sciences","keywords":"Lysis; Green fluorescent protein; Chromatography; Chemistry; Downstream processing; Escherichia coli; Affinity chromatography; Micelle; Dilution; Extraction (chemistry); Aqueous two-phase system; Fluorescence; Cell disruption; Aqueous solution; Biochemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.000120781,0.0003168792,0.0003568382,0.0001320426,0.0001889099,0.00006381045,0.0001846342,0.0007787179,0.00002049201],"category_scores_gemma":[0.000006629456,0.0003105656,0.00007663781,0.0002223234,0.00006520474,0.00007556026,0.00007493449,0.0004563899,0.00001254936],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001692444,"about_ca_system_score_gemma":0.00003086875,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003203224,"about_ca_topic_score_gemma":0.00008793621,"domain_scores_codex":[0.9985192,0.00002306921,0.0004215555,0.0004623603,0.0001569753,0.0004168777],"domain_scores_gemma":[0.9993355,0.00003041487,0.0001766341,0.0003494812,0.00003221815,0.00007578404],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00009139095,0.00009963811,0.0001308561,0.0002649076,0.00004856272,0.0001557334,0.00001834457,0.0001118136,0.9977793,0.0001140977,0.00000599774,0.001179391],"study_design_scores_gemma":[0.001719054,0.00003673948,0.00002281337,0.0002582864,0.00007400668,0.0001116308,0.0002170032,0.02923546,0.9653999,0.00001461183,0.002539659,0.0003708904],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.995542,0.001224938,0.00149754,0.00008190086,0.00018895,0.0002837746,0.00009854067,0.000763006,0.0003193066],"genre_scores_gemma":[0.9980459,0.00002277916,0.001457168,0.000006905711,0.000221469,0.00003024434,0.00003152139,0.0000437803,0.0001402542],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03237942,"threshold_uncertainty_score":0.9999347,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008196045094961666,"score_gpt":0.2078580151765383,"score_spread":0.1996619700815767,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}