{"id":"W2106203631","doi":"10.1002/bit.20806","title":"Affinity‐tagged green fluorescent protein (GFP) extraction from a clarified <i>E. coli</i> cell lysate using a two‐phase aqueous micellar system","year":2006,"lang":"en","type":"article","venue":"Biotechnology and Bioengineering","topic":"Surfactants and Colloidal Systems","field":"Chemistry","cited_by":33,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"National Institute of General Medical Sciences","keywords":"Lysis; Green fluorescent protein; Chromatography; Chemistry; Downstream processing; Escherichia coli; Affinity chromatography; Micelle; Dilution; Extraction (chemistry); Aqueous two-phase system; Fluorescence; Cell disruption; Aqueous solution; Biochemistry; Enzyme; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002606403,0.000710056,0.0004931032,0.0002050063,0.0002162435,0.0004978841,0.0003896507,0.0005462632,0.000373203],"category_scores_gemma":[0.0002004709,0.0001861386,0.0003770334,0.0002310416,0.0002868916,0.0003706907,0.0003087725,0.0008739313,0.0005146202],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004069839,"about_ca_system_score_gemma":0.0003916613,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006935298,"about_ca_topic_score_gemma":0.000877146,"domain_scores_codex":[0.9997988,0.00003773004,0.00001707497,0.00005313396,0.00005857983,0.00003462105],"domain_scores_gemma":[0.999887,0.00003039831,0.00003355778,0.00001118647,0.0000238327,0.00001397932],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000008663055,0.000006506466,0.00002937989,0.00001608145,0.000001472355,0.000009714846,0.000002928664,0.00001969476,0.999541,0.00002227796,0.000008361115,0.0003340074],"study_design_scores_gemma":[0.000002252959,0.00004068044,0.0002544612,0.000001968901,0.00000364626,0.00005061484,0.000005054342,0.0003243196,0.9988926,0.00001323767,0.0004084193,0.000002720468],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.845964,0.001969189,0.1487063,0.0003487016,0.00007340356,0.0002352601,0.0005666739,0.0005675203,0.001568991],"genre_scores_gemma":[0.8759139,0.002954145,0.1142117,0.0003851812,0.00003323883,0.0002276089,0.001759121,0.0001549112,0.004360232],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.000710056,"threshold_uncertainty_score":0.002952874,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008196045094961666,"score_gpt":0.2078580151765383,"score_spread":0.1996619700815767,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}