{"id":"W2106420203","doi":"10.1093/protein/13.5.339","title":"Study of B72.3 combining sites by molecular modeling and site-directed mutagenesis","year":2000,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":0,"is_retracted":false,"has_abstract":true,"ca_institutions":"Queen's University; Saskatchewan Cancer Agency; University of Saskatchewan","funders":"Medical Research Council; Medical Research Council Canada; Saskatchewan Cancer Agency","keywords":"Immunoglobulin light chain; Alanine scanning; Mutagenesis; Chemistry; Stereochemistry; Side chain; Complementarity determining region; Mutant; Site-directed mutagenesis; Residue (chemistry); Alanine; Heavy chain; Molecular model; Biochemistry; Biology; Amino acid; Genetics; Antibody; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002776455,0.0009022012,0.0007690368,0.000218599,0.0003319356,0.0005035652,0.0006854408,0.0005343507,0.001202147],"category_scores_gemma":[0.0005364078,0.0004206261,0.0004514853,0.0003721137,0.000157237,0.0003406569,0.000197469,0.0005956832,0.0003236771],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005764122,"about_ca_system_score_gemma":0.000518059,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003952072,"about_ca_topic_score_gemma":0.002875646,"domain_scores_codex":[0.999836,0.00003993247,0.000008664249,0.00003111466,0.00005366506,0.00003058326],"domain_scores_gemma":[0.9998752,0.00004458164,0.00003013793,0.000009135984,0.00002310092,0.00001779038],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"simulation_or_modeling","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0007718716,0.0006500477,0.00555109,0.0003833399,0.0001746599,0.001247676,0.0001896965,0.5601681,0.408012,0.007708513,0.0006452585,0.01449763],"study_design_scores_gemma":[0.0001286585,0.0004698649,0.0009770129,0.00001730282,0.00008508743,0.0002214877,0.00008730798,0.9089043,0.08633489,0.0006062849,0.002141488,0.00002642359],"study_design_candidate":"bench_or_experimental","study_design_consensus":null,"genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9773489,0.0002915963,0.02015422,0.00005788093,0.00001063846,0.00005817472,0.0001696047,0.0001916324,0.001717306],"genre_scores_gemma":[0.9733944,0.0003985522,0.02509373,0.00003183099,0.000003125708,0.00007068726,0.0003777577,0.00009611936,0.0005336307],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003952072,"threshold_uncertainty_score":0.007858157,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01830196472942979,"score_gpt":0.2499749076423127,"score_spread":0.2316729429128829,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}