{"id":"W2106731625","doi":"10.1002/pro.249","title":"Controllable protein cleavages through intein fragment complementation","year":2009,"lang":"en","type":"article","venue":"Protein Science","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"","keywords":"Intein; Cleavage (geology); Complementation; Recombinant DNA; Protein engineering; Protein splicing; Chemistry; Protein tag; Biochemistry; Stereochemistry; Biology; Fusion protein; Enzyme; Gene; RNA splicing","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008259282,0.0001810796,0.0001543229,0.00005002053,0.000329673,0.0001292213,0.000541023,0.00007083043,0.0001250753],"category_scores_gemma":[0.0002661433,0.0001604024,0.00005742562,0.0002516499,0.0002118091,0.00003525467,0.0001009532,0.00007149483,0.0001234745],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00003709452,"about_ca_system_score_gemma":0.0001395983,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002684869,"about_ca_topic_score_gemma":0.000004532882,"domain_scores_codex":[0.9981802,0.00007880529,0.0002662748,0.0005648188,0.0004614679,0.0004484633],"domain_scores_gemma":[0.9990979,0.000004847202,0.0001360106,0.0004464542,0.0002179767,0.00009674019],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008169104,0.00005570551,0.00001197907,0.000007407897,0.000004932616,0.00000134798,0.00004567241,0.000006277105,0.9854434,0.003673311,0.0002299051,0.01043834],"study_design_scores_gemma":[0.0004693595,0.0008453023,0.0001121321,0.00004476347,0.000003606052,0.000002580627,0.00006597296,0.00004610833,0.9733244,0.005060167,0.01981348,0.00021215],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9481546,0.0005136958,0.03173212,0.001502457,0.0001566964,0.002644718,0.00001067755,0.00005898763,0.01522609],"genre_scores_gemma":[0.9681991,0.00001454513,0.0291242,0.0006336797,0.000145941,0.0002134902,0.00001060027,0.00001084533,0.001647611],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.02004453,"threshold_uncertainty_score":0.654102,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01750516188224444,"score_gpt":0.2897801156604651,"score_spread":0.2722749537782206,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}