{"id":"W2106731625","doi":"10.1002/pro.249","title":"Controllable protein cleavages through intein fragment complementation","year":2009,"lang":"en","type":"article","venue":"Protein Science","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":true,"ca_institutions":"Dalhousie University","funders":"","keywords":"Intein; Cleavage (geology); Complementation; Recombinant DNA; Protein engineering; Protein splicing; Chemistry; Protein tag; Biochemistry; Stereochemistry; Biology; Fusion protein; Enzyme; Gene; RNA splicing","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002512594,0.0006041091,0.0001869563,0.0002209581,0.0001454649,0.0003089775,0.0004552582,0.0003072028,0.0004650696],"category_scores_gemma":[0.0002467412,0.0001697615,0.0002506136,0.0002160561,0.0005269477,0.0003345719,0.0004135157,0.0007482144,0.000250364],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003788379,"about_ca_system_score_gemma":0.0002768702,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002485107,"about_ca_topic_score_gemma":0.0002827605,"domain_scores_codex":[0.9997382,0.00003826334,0.00002318362,0.00007024408,0.00008922484,0.00004080335],"domain_scores_gemma":[0.9998087,0.00005407551,0.00006564262,0.00002940206,0.0000186367,0.00002354683],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000302528,0.00001361179,0.00008357497,0.00003048528,0.000003503643,0.00004630173,0.00001770434,0.0002788174,0.9966195,0.0008606152,0.00002954706,0.001986195],"study_design_scores_gemma":[0.000004252236,0.0000268095,0.0001737528,0.000001520651,0.000002411785,0.00009090814,0.000005400105,0.0009743569,0.9974638,0.00008567642,0.001167196,0.00000381951],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8863086,0.001235764,0.1072173,0.0001737318,0.00004681095,0.0001214451,0.0003223127,0.00048443,0.004089531],"genre_scores_gemma":[0.9639849,0.001102633,0.03205296,0.00006187733,0.000008117529,0.00007934817,0.0003692,0.00009851354,0.002242527],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0006041091,"threshold_uncertainty_score":0.002748609,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01750516188224444,"score_gpt":0.2897801156604651,"score_spread":0.2722749537782206,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}