{"id":"W2106987176","doi":"10.1038/nprot.2009.16","title":"Optimized immunofluorescence staining protocol to detect the nucleoporin Nup98 in different subcellular compartments","year":2009,"lang":"en","type":"article","venue":"Protocol Exchange","topic":"RNA Research and Splicing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":8,"is_retracted":false,"has_abstract":false,"ca_institutions":"McGill University","funders":"Canadian Institutes of Health Research; Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada","keywords":"Immunofluorescence; Staining; Nucleoporin; Chemistry; Subcellular localization; Protocol (science); Cell biology; Molecular biology; Biology; Pathology; Genetics; Medicine; Cytoplasm; Cell nucleus; Antibody; Nuclear transport","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001537225,0.00202619,0.001304253,0.001599628,0.001502107,0.0007263778,0.001866768,0.001029449,0.01016045],"category_scores_gemma":[0.001298986,0.001220946,0.001062282,0.001471772,0.0005170844,0.0009108029,0.0007559655,0.002990528,0.003665046],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009406544,"about_ca_system_score_gemma":0.001391114,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001897088,"about_ca_topic_score_gemma":0.00542916,"domain_scores_codex":[0.9988794,0.0001912557,0.0002128156,0.0002441919,0.0002976525,0.0001747002],"domain_scores_gemma":[0.9987379,0.0004087111,0.0001051656,0.0002797703,0.0003713924,0.00009716668],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"not_applicable","study_design_scores_codex":[0.0001423532,0.00006483815,0.0001392473,0.0002077924,0.00002454477,0.0001088578,0.00005804167,0.0002359953,0.9920565,0.000656472,0.000756696,0.005548486],"study_design_scores_gemma":[0.00008795882,0.0001163479,0.001641745,0.00003931819,0.0001097313,0.0005895499,0.00003438353,0.002430186,0.9752697,0.0005014886,0.01911393,0.00006551523],"study_design_candidate":"not_applicable","study_design_consensus":null,"genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.2716036,0.002463948,0.6947661,0.0009056788,0.0008793128,0.00538777,0.006761971,0.007194364,0.01003725],"genre_scores_gemma":[0.1013677,0.002958014,0.8459725,0.0005407496,0.0001041959,0.009134227,0.01447759,0.002443691,0.0230013],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.01016045,"threshold_uncertainty_score":0.03399009,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02444885415999526,"score_gpt":0.3345136749387401,"score_spread":0.3100648207787449,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}