{"id":"W2107074652","doi":"10.1002/bab.1238","title":"A simple, rapid one‐step <scp>ELISA</scp> using antibody–antibody complex","year":2014,"lang":"en","type":"article","venue":"Biotechnology and Applied Biochemistry","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":6,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Science Fund for Distinguished Young Scholars; National Natural Science Foundation of China","keywords":"Chemistry; Antibody; Adsorption; Antigen; Chromatography; Two step; Primary and secondary antibodies; Combinatorial chemistry; Biology; Immunology; Organic chemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0008694043,0.001675516,0.001286598,0.0009343472,0.0005790361,0.0008664883,0.001892945,0.00149672,0.003099685],"category_scores_gemma":[0.001152753,0.0007191144,0.0008967235,0.0006610716,0.0006710655,0.001096349,0.0009859444,0.001779823,0.00361995],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004341928,"about_ca_system_score_gemma":0.0008570648,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005169531,"about_ca_topic_score_gemma":0.000925194,"domain_scores_codex":[0.9977399,0.0003388338,0.0001426197,0.0006282628,0.001021063,0.0001293138],"domain_scores_gemma":[0.9991177,0.0002471257,0.0001269495,0.0001430051,0.0002824128,0.00008288506],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0000516016,0.000101393,0.0002550843,0.0005412425,0.0000320081,0.0001227148,0.0000207262,0.000147552,0.9720585,0.0005348113,0.001715364,0.02441913],"study_design_scores_gemma":[0.00002562864,0.0003702337,0.001492629,0.00002831463,0.00003294261,0.001223269,0.00001852462,0.002720782,0.9764234,0.0002574548,0.01734737,0.00005941546],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.1205608,0.01308343,0.84057,0.001038312,0.001479005,0.003766518,0.0015122,0.00657095,0.0114188],"genre_scores_gemma":[0.2745333,0.008725325,0.6869307,0.001760256,0.0004396768,0.002416978,0.003947171,0.0004347248,0.02081195],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.003099685,"threshold_uncertainty_score":0.01036948,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01249263790154082,"score_gpt":0.2763989158504397,"score_spread":0.2639062779488989,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}