{"id":"W2107650081","doi":"10.1006/jmbi.2000.3722","title":"Hydrogen bonding and catalysis: a novel explanation for how a single amino acid substitution can change the ph optimum of a glycosidase 1 1Edited by M. F. Summers","year":2000,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Carbohydrate Chemistry and Synthesis","field":"Chemistry","cited_by":229,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Chemistry; Asparagine; Stereochemistry; Residue (chemistry); Active site; Aspartic acid; Protonation; Deprotonation; Titration; Catalysis; Amino acid; Enzyme; Organic chemistry; Biochemistry","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001822043,0.0001241185,0.0002439674,0.00005512675,0.00006092527,0.00001755843,0.0001419868,0.0001466558,0.00003458087],"category_scores_gemma":[0.0001199115,0.0000949399,0.0001420308,0.000102062,0.0001132468,0.00005749372,0.00001432863,0.0001052363,1.289317e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00005971598,"about_ca_system_score_gemma":0.00002694589,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00002716582,"about_ca_topic_score_gemma":0.000007018427,"domain_scores_codex":[0.9993136,0.00002341563,0.0002619468,0.0001394437,0.00009716547,0.0001644133],"domain_scores_gemma":[0.9993185,0.00007163587,0.0003243542,0.0001324837,0.00008848297,0.00006451835],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001306794,0.00007660904,0.00006135533,0.00003951077,0.0001605274,0.000008438115,0.0001775347,0.00000672835,0.9969929,0.00001983493,0.00006025823,0.002265582],"study_design_scores_gemma":[0.0007036884,0.00007132084,0.000004662109,0.00004520214,0.0001821794,0.0001350453,0.0002090821,0.0002714497,0.995877,0.00003860566,0.002364216,0.00009754363],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9960198,0.001627175,0.0006817418,0.001341117,0.00002573873,0.00006260529,0.0001506842,0.000004872576,0.00008629897],"genre_scores_gemma":[0.9993693,0.00009529347,0.0001542542,0.00007332055,0.0001084194,0.00001716262,0.0001256391,0.00001013122,0.00004647259],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.00334954,"threshold_uncertainty_score":0.3871537,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02379945794566215,"score_gpt":0.2414852040217281,"score_spread":0.2176857460760659,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}