{"id":"W2107711768","doi":"10.1128/aem.01930-08","title":"Helix α4 of the <i>Bacillus thuringiensis</i> Cry1Aa Toxin Plays a Critical Role in the Postbinding Steps of Pore Formation","year":2008,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Fonds Québécois de la Recherche sur la Nature et les Technologies","keywords":"Mutant; Bacillus thuringiensis; Dithiothreitol; Biochemistry; Biophysics; Spodoptera; Alpha helix; Helix (gastropod); Biology; Protein structure; Circular dichroism; Chemistry; Cell biology; Enzyme; Bacteria; Recombinant DNA; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000872895,0.00009236738,0.0001098115,0.000017034,0.00007290025,0.000001832807,0.0001298195,0.0001129408,0.000007459707],"category_scores_gemma":[0.000006388084,0.00006141753,0.00004343766,0.0000318749,0.0003515057,0.000002134914,0.0001009186,0.00007004593,0.000001295776],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000008526148,"about_ca_system_score_gemma":0.000008709787,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000005576379,"about_ca_topic_score_gemma":0.00001791318,"domain_scores_codex":[0.9994482,0.00004078101,0.0001812676,0.0001396503,0.00003736495,0.0001527042],"domain_scores_gemma":[0.9997332,0.00002114767,0.00005846023,0.0001712936,0.000002765151,0.00001318618],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003728366,0.00005423123,0.002622219,0.00001239586,0.000007416023,6.769629e-7,0.0004166244,0.0000116382,0.996367,0.0002432796,0.00008053151,0.0001467027],"study_design_scores_gemma":[0.0003012381,0.0001182568,0.01323031,0.000007602289,0.00000863126,0.0001081079,0.0009595208,0.000003790468,0.9831414,0.0001254943,0.001911056,0.00008465936],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.998602,0.0004275566,0.00003050893,0.00008270046,0.00002184156,0.0001472593,0.00006705645,0.000001025735,0.0006200603],"genre_scores_gemma":[0.9991267,0.0003557228,0.00008706509,0.0002951042,0.00002053378,0.000009527987,0.00008385078,0.000005842717,0.00001561151],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01322567,"threshold_uncertainty_score":0.2504534,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004036116717552832,"score_gpt":0.1733964244591482,"score_spread":0.1693603077415954,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}