{"id":"W2107711768","doi":"10.1128/aem.01930-08","title":"Helix α4 of the <i>Bacillus thuringiensis</i> Cry1Aa Toxin Plays a Critical Role in the Postbinding Steps of Pore Formation","year":2008,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":19,"is_retracted":false,"has_abstract":true,"ca_institutions":"Biotechnology Research Institute; Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Fonds Québécois de la Recherche sur la Nature et les Technologies","keywords":"Mutant; Bacillus thuringiensis; Dithiothreitol; Biochemistry; Biophysics; Spodoptera; Alpha helix; Helix (gastropod); Biology; Protein structure; Circular dichroism; Chemistry; Cell biology; Enzyme; Bacteria; Recombinant DNA; Genetics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001160794,0.0003660301,0.0001919045,0.0001104179,0.0001448273,0.0001813339,0.0001630898,0.0002096839,0.0007101978],"category_scores_gemma":[0.0001628055,0.000121433,0.000277459,0.0001119311,0.0001981166,0.0001378751,0.0001417899,0.0003466561,0.0003958406],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003078687,"about_ca_system_score_gemma":0.0001765855,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0008350362,"about_ca_topic_score_gemma":0.0008135654,"domain_scores_codex":[0.9998927,0.00002175625,0.00001157902,0.00002033626,0.00003429096,0.00001926062],"domain_scores_gemma":[0.999886,0.00001407271,0.00005614931,0.00001009559,0.0000142612,0.00001934884],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007806847,0.000008401367,0.0004800822,0.00002162102,0.000003664753,0.00004080738,0.000008215122,0.00005590445,0.9986874,0.00003241481,0.00002123525,0.000562088],"study_design_scores_gemma":[0.00001322398,0.0002800659,0.009710439,0.000008421282,0.00001403416,0.0003343117,0.00002352173,0.0004680273,0.9876008,0.00003491783,0.00150686,0.0000053708],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9976816,0.0004526956,0.0009984333,0.00004510836,0.00001889591,0.00001835842,0.0001624303,0.00002559117,0.0005967612],"genre_scores_gemma":[0.9960871,0.0003038341,0.001814798,0.00004840283,0.000007735322,0.00001224061,0.0006062665,0.00001075917,0.001108954],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0008350362,"threshold_uncertainty_score":0.002375841,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004036116717552832,"score_gpt":0.1733964244591482,"score_spread":0.1693603077415954,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}