{"id":"W2107871391","doi":"10.1002/0471142301.ns0523s34","title":"Monitoring Protein‐Protein Interactions in Living Cells by Bioluminescence Resonance Energy Transfer (<scp>BRET</scp>)","year":2006,"lang":"en","type":"article","venue":"Current Protocols in Neuroscience","topic":"Receptor Mechanisms and Signaling","field":"Biochemistry, Genetics and Molecular Biology","cited_by":99,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"","keywords":"Bioluminescence; Luciferase; Green fluorescent protein; Förster resonance energy transfer; Chemistry; Energy transfer; Biophysics; Protein–protein interaction; G protein-coupled receptor; Cell biology; Receptor; Fluorescence; Biochemistry; Transfection; Biology; Physics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.0003176267,0.0002523008,0.0001838596,0.0001175956,0.0001237338,0.0001156719,0.0005228696,0.00008230002,0.000004415547],"category_scores_gemma":[0.0001592256,0.0002592127,0.00006921315,0.0005086572,0.0001172795,0.000044561,0.000126105,0.0002721752,0.000001918282],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004542776,"about_ca_system_score_gemma":0.00008220116,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00009460565,"about_ca_topic_score_gemma":0.0000357035,"domain_scores_codex":[0.997773,0.0001420642,0.0004497554,0.0008144401,0.0002765471,0.0005441887],"domain_scores_gemma":[0.9994326,0.0000258146,0.0000865025,0.0003243202,0.00005143698,0.00007937136],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000007712102,0.0002409624,0.0034368,0.00004067987,2.703773e-7,0.000003100123,0.00002183623,0.00008046484,0.993058,0.0001639014,0.00009102032,0.002855282],"study_design_scores_gemma":[0.0002469392,0.0001296367,0.001254974,0.0008431041,7.431922e-7,0.000002542547,0.0000146694,0.0003530768,0.9516903,0.00006980878,0.04525274,0.0001414473],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9606587,0.0006175895,0.02406577,0.00001480047,0.0005179867,0.01393428,0.00001707018,0.00003078557,0.0001430033],"genre_scores_gemma":[0.9662607,0.00004877372,0.0002348045,0.0000189624,0.0001864769,0.03267733,0.000003832136,0.00002578722,0.0005433248],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04516172,"threshold_uncertainty_score":0.999986,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02252414751446555,"score_gpt":0.2913050526515669,"score_spread":0.2687809051371013,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}