{"id":"W2108513111","doi":"10.1128/aem.68.6.3169-3171.2002","title":"Rapid Detection of <i>Escherichia coli</i> O157:H7 with Multiplex Real-Time PCR Assays","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":130,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Escherichia coli; STX2; SYBR Green I; Primer (cosmetics); Melting curve analysis; Shiga toxin; Multiplex; Multiplex polymerase chain reaction; Biology; Real-time polymerase chain reaction; Polymerase chain reaction; Enterobacteriaceae; Molecular biology; Microbiology; Gene; Chemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001181068,0.001179916,0.001010119,0.0007050057,0.0003087458,0.0008557502,0.0006488483,0.0006234029,0.002813614],"category_scores_gemma":[0.001442188,0.0006382387,0.0004794676,0.0005835537,0.000564957,0.0007508343,0.0006479797,0.001207384,0.001512943],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003524181,"about_ca_system_score_gemma":0.0003109681,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001278597,"about_ca_topic_score_gemma":0.0003049367,"domain_scores_codex":[0.9989304,0.0001980828,0.00008723283,0.0002990337,0.0003159341,0.0001692284],"domain_scores_gemma":[0.9993956,0.0001891372,0.000116896,0.00005794149,0.0001566936,0.00008373131],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008148853,0.00005489236,0.0002558289,0.0000663372,0.000003938103,0.00002805344,0.00003133249,0.0000565666,0.99625,0.0001116835,0.0001547703,0.002905182],"study_design_scores_gemma":[0.00002972882,0.0004205694,0.001311901,0.00002130649,0.00001890414,0.0001766012,0.00004074103,0.001848296,0.9929559,0.0001910839,0.00296765,0.00001725745],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.5175877,0.00243353,0.4681741,0.0003956629,0.0003615014,0.0008276107,0.002135855,0.002519909,0.005564245],"genre_scores_gemma":[0.4616474,0.002622822,0.5176736,0.0003795934,0.0001597704,0.002219362,0.004066148,0.0003665911,0.0108647],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002813614,"threshold_uncertainty_score":0.009412467,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006178496765277332,"score_gpt":0.1780862536302605,"score_spread":0.1719077568649831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}