{"id":"W2108513111","doi":"10.1128/aem.68.6.3169-3171.2002","title":"Rapid Detection of <i>Escherichia coli</i> O157:H7 with Multiplex Real-Time PCR Assays","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Escherichia coli research studies","field":"Biochemistry, Genetics and Molecular Biology","cited_by":130,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"","keywords":"Escherichia coli; STX2; SYBR Green I; Primer (cosmetics); Melting curve analysis; Shiga toxin; Multiplex; Multiplex polymerase chain reaction; Biology; Real-time polymerase chain reaction; Polymerase chain reaction; Enterobacteriaceae; Molecular biology; Microbiology; Gene; Chemistry; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008718151,0.0001936566,0.0002246991,0.00002717551,0.0001026408,0.000005263279,0.0001039302,0.000162909,0.0000897595],"category_scores_gemma":[0.000005801021,0.0001645085,0.00003992426,0.00005065639,0.0005112632,0.000002928003,0.0001714073,0.000104185,0.00002758599],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001878366,"about_ca_system_score_gemma":0.000005427591,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0000201686,"about_ca_topic_score_gemma":0.00001383496,"domain_scores_codex":[0.9990585,0.0000536771,0.0001688977,0.0003813353,0.00004794096,0.0002896168],"domain_scores_gemma":[0.9996116,0.00002260908,0.00008324459,0.0002124036,0.000005879824,0.00006422808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001314355,0.0001183801,0.001131432,0.00001441539,0.0001040213,0.000001306039,0.0000560698,0.000006090277,0.9954826,0.000006033331,0.0002760583,0.002672189],"study_design_scores_gemma":[0.001317926,0.0008277899,0.006835963,0.000004797134,0.00002899645,0.00002778014,0.000157538,0.00006317117,0.9641935,0.000004716695,0.02627141,0.000266383],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9958056,0.0008072355,0.00006357465,0.0000327761,0.00001908235,0.0002797786,0.00006019872,0.00001188523,0.002919933],"genre_scores_gemma":[0.9953492,0.003250296,0.0006420444,0.0001251899,0.00005026106,0.00005392131,0.0001194851,0.00002425612,0.0003853365],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03128904,"threshold_uncertainty_score":0.6708464,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006178496765277332,"score_gpt":0.1780862536302605,"score_spread":0.1719077568649831,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}