{"id":"W2108964051","doi":"10.1016/j.jmb.2010.07.036","title":"Cysteine-to-Serine Mutants Dramatically Reorder the Active Site of Human ABO(H) Blood Group B Glycosyltransferase without Affecting Activity: Structural Insights into Cooperative Substrate Binding","year":2010,"lang":"en","type":"article","venue":"Journal of Molecular Biology","topic":"Glycosylation and Glycoproteins Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":false,"ca_institutions":"Institute for Biological Sciences; University of Ottawa; University of Alberta; University of Victoria","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research; Michael Smith Health Research BC","keywords":"Active site; Chemistry; Stereochemistry; Cooperativity; Binding site; Moiety; Glycosyltransferase; Dimer; Mutant; Cysteine; Substrate (aquarium); Cooperative binding; Enzyme; Biochemistry; Biology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002476866,0.00058718,0.0004691591,0.0001553105,0.000230924,0.0005618216,0.0006524405,0.0006421816,0.001676653],"category_scores_gemma":[0.000292531,0.0003604119,0.0004619752,0.0002261179,0.0004790022,0.0003525765,0.0002253053,0.001120727,0.0008700921],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000646809,"about_ca_system_score_gemma":0.0004933456,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001888037,"about_ca_topic_score_gemma":0.002471271,"domain_scores_codex":[0.9998094,0.0000362601,0.00003275609,0.00003189654,0.00004787293,0.00004176858],"domain_scores_gemma":[0.999626,0.00009361519,0.0001037295,0.00004574871,0.00003143171,0.00009942313],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004807055,0.00008771529,0.0003237032,0.00003496291,0.00003092796,0.0003243846,0.00003432341,0.000456803,0.9966745,0.000350173,0.00009757104,0.001104179],"study_design_scores_gemma":[0.00007436963,0.0001656374,0.001056895,0.00000484802,0.00003749564,0.0006693021,0.0000399729,0.002035465,0.9947668,0.00008175988,0.001052861,0.00001466181],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9972878,0.0003094408,0.00157047,0.00009979078,0.00005948684,0.0000192673,0.0001140066,0.00006305789,0.0004767917],"genre_scores_gemma":[0.9969245,0.0001801844,0.001625746,0.0000777356,0.00001399846,0.0000100989,0.0001858215,0.00005478795,0.0009271974],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001888037,"threshold_uncertainty_score":0.005608976,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.00752587234783618,"score_gpt":0.3070367233547978,"score_spread":0.2995108510069616,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}