{"id":"W2109115572","doi":"10.1128/aem.02662-06","title":"Use of PCR-Targeted Mutagenesis To Disrupt Production of Fusaricidin-Type Antifungal Antibiotics in <i>Paenibacillus polymyxa</i>","year":2007,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Bacterial biofilms and quorum sensing","field":"Biochemistry, Genetics and Molecular Biology","cited_by":66,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Paenibacillus polymyxa; Biology; Leptosphaeria maculans; Nonribosomal peptide; Microbiology; Genetics; Mutagenesis; Gene; Mutant; Bacteria; Biosynthesis","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001344018,0.0001453468,0.0002195155,0.00006704433,0.00002956605,0.000003521571,0.00005545655,0.0001870631,0.000006866993],"category_scores_gemma":[0.00001139864,0.0001401231,0.00003318026,0.00008938789,0.0001859399,0.000003536108,0.000119432,0.00005187893,0.000002262867],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001232459,"about_ca_system_score_gemma":0.000008412842,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004306613,"about_ca_topic_score_gemma":0.00003033292,"domain_scores_codex":[0.999086,0.00002160888,0.000297053,0.0003280048,0.0000283122,0.0002390412],"domain_scores_gemma":[0.9996559,0.00001074933,0.00009785217,0.0001813417,0.000006321317,0.00004780534],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003616177,0.0001076925,0.01905742,0.00001391715,0.00002451962,0.000001595341,0.00005073463,0.00001698303,0.9776853,0.00001664247,0.00004408183,0.002619476],"study_design_scores_gemma":[0.0002383373,0.000235352,0.05158279,0.000006411191,0.00001336167,0.00002695437,0.0001097677,7.716259e-7,0.9454826,0.000001324319,0.002161971,0.0001403528],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9992295,0.0001924811,0.00003460571,0.00002308086,0.0002120391,0.0001994093,0.0000669226,0.000003228237,0.00003876321],"genre_scores_gemma":[0.9981711,0.0001415805,0.001128746,0.0001103216,0.00005402174,2.388119e-7,0.0003268858,0.00001501117,0.00005210015],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.03252537,"threshold_uncertainty_score":0.5714054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.008352990882778879,"score_gpt":0.1962489506174829,"score_spread":0.187895959734704,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}