{"id":"W2111661569","doi":"10.1002/pro.2223","title":"Rapid expression screening of eukaryotic membrane proteins in <i>Pichia pastoris</i>","year":2013,"lang":"en","type":"article","venue":"Protein Science","topic":"Cellular transport and secretion","field":"Biochemistry, Genetics and Molecular Biology","cited_by":28,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Canadian Institutes of Health Research; Alberta Heritage Foundation for Medical Research; Fondation pour la Recherche Médicale; Canada Research Chairs; Alberta Innovates; Alberta Innovates - Health Solutions","keywords":"Pichia pastoris; Biology; Membrane protein; Vesicle-associated membrane protein 8; Heterologous; Green fluorescent protein; Heterologous expression; Cell biology; Molecular biology; Gene; Recombinant DNA; Biochemistry; Membrane","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.001062825,0.001232996,0.001392828,0.0008083174,0.0003674296,0.0007484758,0.0008273394,0.0005193155,0.001384475],"category_scores_gemma":[0.0006640121,0.0004867194,0.0007274828,0.001278395,0.0003080613,0.0005137757,0.000786972,0.0009273425,0.002291711],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004854854,"about_ca_system_score_gemma":0.0002953517,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001054865,"about_ca_topic_score_gemma":0.001554544,"domain_scores_codex":[0.9989372,0.0002616872,0.0001663559,0.0001471144,0.0003883597,0.00009934931],"domain_scores_gemma":[0.9996632,0.0001151294,0.00005186465,0.00006929603,0.00006225569,0.00003834935],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003635625,0.00003266093,0.0001146426,0.00008608976,0.00001052391,0.00007722697,0.00002046766,0.00009299178,0.9969031,0.00009358671,0.0002030513,0.002329141],"study_design_scores_gemma":[0.00001452166,0.0001877911,0.001586215,0.00001054878,0.00003258661,0.0002187387,0.00001944021,0.00139674,0.9901523,0.00006245269,0.006304483,0.00001414976],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6930716,0.005410391,0.264282,0.0009630254,0.0003201176,0.0020996,0.01726854,0.004334187,0.01225056],"genre_scores_gemma":[0.586396,0.008759684,0.3350849,0.0003644567,0.00008456341,0.001866153,0.04285971,0.001177814,0.02340667],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001392828,"threshold_uncertainty_score":0.005620837,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01047019390862121,"score_gpt":0.2149398973532189,"score_spread":0.2044697034445977,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}