{"id":"W2112264688","doi":"10.1128/aem.67.4.1700-1709.2001","title":"Molecular Characterization of a Theta Replication Plasmid and Its Use for Development of a Two-Component Food-Grade Cloning System for <i>Lactococcus lactis</i>","year":2001,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Probiotics and Fermented Foods","field":"Agricultural and Biological Sciences","cited_by":70,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Agriculture and Agri-Food Canada; Natural Sciences and Engineering Research Council of Canada; Novalait; Lallemand; Dairy Farmers of Canada","keywords":"Replicon; Plasmid; Biology; Lactococcus lactis; Genetics; Gene; Cloning (programming); Cloning vector; Vector (molecular biology); Recombinant DNA; Bacteria","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008898017,0.0001020882,0.000170254,0.00001062473,0.00008254137,0.000007596225,0.00005594363,0.0000743943,0.000001733976],"category_scores_gemma":[0.000001819181,0.00005366037,0.00002929449,0.00002875665,0.00004498926,0.00002914753,0.00005106168,0.00002665441,2.638585e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001939965,"about_ca_system_score_gemma":0.000002185388,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000002509979,"about_ca_topic_score_gemma":0.000003035511,"domain_scores_codex":[0.9993511,0.0000120759,0.000228889,0.0002499954,0.00002123077,0.0001367537],"domain_scores_gemma":[0.999715,0.00004624617,0.0001601996,0.00004286637,0.000005442992,0.00003020532],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008285727,0.00007062004,0.0005133164,0.00003194104,0.00002891734,7.43139e-8,0.00006073617,0.000001837457,0.9945498,0.001056117,6.008669e-7,0.003603221],"study_design_scores_gemma":[0.0005979365,0.0002628705,0.01091481,0.00001890947,0.00002247676,0.0000162568,0.00008964934,0.0001087046,0.9839116,0.00003417278,0.003906953,0.0001156379],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9984313,0.00005636024,0.0003167494,0.00004765174,0.0000190949,0.0008276184,0.0002847716,0.000008537239,0.000007888701],"genre_scores_gemma":[0.998457,0.00004871649,0.0004086503,0.00003910908,0.00001169979,0.0001051023,0.0009236978,0.000001868613,0.00000414196],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01063814,"threshold_uncertainty_score":0.2188206,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01761927367185273,"score_gpt":0.1967587572671722,"score_spread":0.1791394835953194,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}