{"id":"W2112632935","doi":"10.3791/3422","title":"Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; Hospital for Sick Children; Multiple Sclerosis Society; Natural Sciences and Engineering Research Council of Canada; Multiple Sclerosis Society of Canada","keywords":"Cell biology; Caspase; Oligodendrocyte; Biology; Programmed cell death; Apoptosis; Population; Neuroscience; Myelin; Central nervous system; Biochemistry; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003181098,0.0002067718,0.0003810437,0.0001085354,0.0001249343,0.00004102712,0.00008380196,0.0001647876,0.00001452347],"category_scores_gemma":[0.00003596034,0.0002027568,0.0001439546,0.00007659673,0.00004104673,0.00006489421,0.00007631915,0.0001295131,7.097789e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002688798,"about_ca_system_score_gemma":0.00005340683,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001044775,"about_ca_topic_score_gemma":9.618268e-7,"domain_scores_codex":[0.998637,0.0002067929,0.0004616439,0.0002020239,0.0002021168,0.0002904211],"domain_scores_gemma":[0.9990435,0.00002487242,0.0005000107,0.000138291,0.00009781103,0.0001955714],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0004946829,0.0001810527,0.01670199,0.00002740736,0.0001254022,0.000005157722,0.0004262999,0.000006969323,0.9818134,0.000002043584,0.000005485079,0.000210154],"study_design_scores_gemma":[0.002618588,0.0002047958,0.000885133,0.00004575594,0.0001478198,0.00008184849,0.0006996311,0.0001131095,0.9949946,0.000001510867,0.00002778552,0.0001794447],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9951829,0.002577045,0.0009048363,0.000004100159,0.001129758,0.0001445986,0.000004664167,0.000005393039,0.00004671763],"genre_scores_gemma":[0.9950743,0.0003127267,0.004175367,0.00001672344,0.0003626597,0.000002947799,0.000005146573,0.00003174597,0.00001843393],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01581685,"threshold_uncertainty_score":0.8268181,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02759259788105186,"score_gpt":0.3514119372181702,"score_spread":0.3238193393371183,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}