{"id":"W2112632935","doi":"10.3791/3422","title":"Monitoring Cleaved Caspase-3 Activity and Apoptosis of Immortalized Oligodendroglial Cells using Live-cell Imaging and Cleaveable Fluorogenic-dye Substrates Following Potassium-induced Membrane Depolarization","year":2012,"lang":"en","type":"article","venue":"Journal of Visualized Experiments","topic":"Mitochondrial Function and Pathology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Guelph","funders":"Canadian Institutes of Health Research; Hospital for Sick Children; Multiple Sclerosis Society; Natural Sciences and Engineering Research Council of Canada; Multiple Sclerosis Society of Canada","keywords":"Cell biology; Caspase; Oligodendrocyte; Biology; Programmed cell death; Apoptosis; Population; Neuroscience; Myelin; Central nervous system; Biochemistry; Medicine","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004737773,0.0004884157,0.0003185706,0.0006606153,0.0002726579,0.0004052153,0.0005281405,0.0004012281,0.0008834894],"category_scores_gemma":[0.000230579,0.0001832771,0.0003359394,0.0006889642,0.0003206505,0.000370572,0.000298011,0.0008690965,0.0003922921],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003915146,"about_ca_system_score_gemma":0.0003133618,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009738379,"about_ca_topic_score_gemma":0.001634677,"domain_scores_codex":[0.9995738,0.00005064409,0.00006845777,0.00008630787,0.0001413415,0.00007938619],"domain_scores_gemma":[0.9997824,0.00004227825,0.00006462428,0.00002966278,0.00004598219,0.000035081],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005246063,0.00001902985,0.00014258,0.000028106,0.000002973633,0.00004198387,0.00003601003,0.00003084386,0.9988783,0.00007131603,0.00001624184,0.0006801222],"study_design_scores_gemma":[0.000007883452,0.00009119546,0.002086787,0.000004925571,0.000007831044,0.0001879037,0.00002673217,0.0006913259,0.9959638,0.00004780349,0.0008784991,0.000005233532],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9312199,0.003856868,0.059378,0.0001067964,0.0000856995,0.0002212713,0.001570875,0.0004152562,0.00314539],"genre_scores_gemma":[0.8829179,0.005749876,0.1011608,0.000124691,0.0000335903,0.0007341845,0.003380389,0.00009286164,0.005805754],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009738379,"threshold_uncertainty_score":0.002955556,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02759259788105186,"score_gpt":0.3514119372181702,"score_spread":0.3238193393371183,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}