{"id":"W2113274575","doi":"10.2144/000112455","title":"Yeast Split-Ubiquitin-Based Cytosolic Screening System to Detect Interactions Between Transcriptionally Active Proteins","year":2007,"lang":"en","type":"article","venue":"BioTechniques","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":81,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Two-hybrid screening; Yeast; Protein–protein interaction; Biology; Ubiquitin; Cytosol; Complementary DNA; cDNA library; Bimolecular fluorescence complementation; Computational biology; Saccharomyces cerevisiae; Cytoplasm; Transcription factor; Cell biology; Biochemistry; Genetics; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000519725,0.000649827,0.0006102438,0.0008034445,0.0002960169,0.0004596749,0.0005784729,0.0003813604,0.001536068],"category_scores_gemma":[0.0002662732,0.0002626281,0.0003800867,0.000476614,0.00034891,0.0002797076,0.0005501974,0.0008964707,0.001015886],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003773716,"about_ca_system_score_gemma":0.0003622594,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000396497,"about_ca_topic_score_gemma":0.0006990463,"domain_scores_codex":[0.9995413,0.00009157384,0.00003715866,0.0001104264,0.0001726738,0.00004690468],"domain_scores_gemma":[0.9996002,0.0001135503,0.00006665468,0.00007190133,0.00006444567,0.00008321242],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004838282,0.00003183359,0.0001901065,0.00002594324,0.000005968362,0.00002491499,0.000006120488,0.00006185637,0.997738,0.0001517846,0.00008055499,0.001634505],"study_design_scores_gemma":[0.00001326562,0.0001142374,0.00102864,0.000002616214,0.00001161969,0.0002420194,0.000009574606,0.002492686,0.9948317,0.00006255193,0.001184479,0.000006569226],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.6325668,0.001618198,0.3571773,0.0003167678,0.0001321209,0.0003017648,0.002139575,0.002557378,0.003189983],"genre_scores_gemma":[0.8444178,0.001245629,0.1452072,0.000178272,0.00003215006,0.0003811906,0.003841375,0.000196881,0.004499628],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001536068,"threshold_uncertainty_score":0.005138695,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02644267493008941,"score_gpt":0.3154480382252524,"score_spread":0.289005363295163,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}