{"id":"W2113274575","doi":"10.2144/000112455","title":"Yeast Split-Ubiquitin-Based Cytosolic Screening System to Detect Interactions Between Transcriptionally Active Proteins","year":2007,"lang":"en","type":"article","venue":"BioTechniques","topic":"Fungal and yeast genetics research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":81,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"","keywords":"Two-hybrid screening; Yeast; Protein–protein interaction; Biology; Ubiquitin; Cytosol; Complementary DNA; cDNA library; Bimolecular fluorescence complementation; Computational biology; Saccharomyces cerevisiae; Cytoplasm; Transcription factor; Cell biology; Biochemistry; Genetics; Gene; Enzyme","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004086224,0.00019165,0.00016065,0.0001960453,0.0001462561,0.00004284543,0.0002762902,0.0002189517,0.00001843875],"category_scores_gemma":[0.0000493319,0.000187813,0.0001316827,0.0002195281,0.00006036752,0.000005170245,0.00008579309,0.0002245601,0.00002001665],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00006718501,"about_ca_system_score_gemma":0.00009254807,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001112834,"about_ca_topic_score_gemma":0.0001482587,"domain_scores_codex":[0.9985909,0.00007000995,0.0002593125,0.000408237,0.0002682796,0.000403236],"domain_scores_gemma":[0.9992024,0.00001994881,0.00005759418,0.0003188465,0.0001828874,0.0002183152],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.000231503,0.00002289657,0.0002129026,0.00004182192,0.0000348639,0.000004772124,0.00002790271,0.00001308373,0.9746121,0.00005775946,0.0001842057,0.02455621],"study_design_scores_gemma":[0.0001660333,0.0004778097,0.007077354,0.000110378,0.00001182137,0.000009069505,0.00005600904,0.00003276391,0.9569456,0.000008184616,0.03489181,0.0002131443],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"empirical","genre_scores_codex":[0.4964852,0.0000705167,0.4994261,0.0002733009,0.00005096103,0.0008145591,0.0001873938,0.0001418459,0.002550159],"genre_scores_gemma":[0.9513589,0.000005716936,0.04751903,0.0001293522,0.0004706517,0.0001134861,0.0001090508,0.00003689416,0.00025691],"genre_candidate":"empirical","genre_consensus":null,"teacher_disagreement_score":0.4548737,"threshold_uncertainty_score":0.7658792,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02644267493008941,"score_gpt":0.3154480382252524,"score_spread":0.289005363295163,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}