{"id":"W2113371439","doi":"10.1016/j.pep.2003.08.005","title":"Overexpression and purification of untagged polynucleotide phosphorylases","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Phosphorolysis; Polynucleotide phosphorylase; Biochemistry; Polynucleotide; Enzyme; Nuclease; Escherichia coli; Biology; Streptomyces coelicolor; Chemistry; Streptomyces; Affinity chromatography; Molecular biology; Gene; Bacteria; Mutant; Purine nucleoside phosphorylase; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002144936,0.0001272125,0.0001252731,0.00005364146,0.0001087563,0.00002073323,0.00005811255,0.0001336292,0.000004602756],"category_scores_gemma":[0.000165898,0.0001046803,0.0000345069,0.00007844787,0.000106924,0.0000126337,0.00003019234,0.00006093504,5.32117e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004935326,"about_ca_system_score_gemma":0.00001730044,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000721681,"about_ca_topic_score_gemma":8.271157e-7,"domain_scores_codex":[0.9990965,0.0001133288,0.0002285277,0.0003358155,0.0001150766,0.000110736],"domain_scores_gemma":[0.9993526,0.00001021444,0.0001998863,0.0002920143,0.0000815256,0.0000637519],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00006226295,0.00005258706,0.0004374222,0.00002732439,0.000006280453,1.405208e-7,0.00002485586,5.213046e-7,0.9929941,0.0005698315,0.000134702,0.005690007],"study_design_scores_gemma":[0.0002331314,0.00008666924,0.0009823698,0.0001001569,0.00001036238,0.000005810283,0.00008341592,0.00002106737,0.9905965,0.0005200634,0.007230026,0.0001303759],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9935784,0.00147855,0.004026453,0.00008936037,0.00001346655,0.0003299657,0.00001062511,0.00002688497,0.0004462937],"genre_scores_gemma":[0.9826948,0.0006409572,0.01619567,0.00002953236,0.00002237267,0.00003252108,0.00005405791,0.00001243878,0.0003176264],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01216922,"threshold_uncertainty_score":0.426874,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009574818329172707,"score_gpt":0.2540293732801535,"score_spread":0.2444545549509808,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}