{"id":"W2113371439","doi":"10.1016/j.pep.2003.08.005","title":"Overexpression and purification of untagged polynucleotide phosphorylases","year":2003,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":false,"ca_institutions":"University of British Columbia","funders":"","keywords":"Phosphorolysis; Polynucleotide phosphorylase; Biochemistry; Polynucleotide; Enzyme; Nuclease; Escherichia coli; Biology; Streptomyces coelicolor; Chemistry; Streptomyces; Affinity chromatography; Molecular biology; Gene; Bacteria; Mutant; Purine nucleoside phosphorylase; Genetics","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004113335,0.0007014601,0.0005265348,0.0003595712,0.0004771724,0.0007269318,0.0008178572,0.0004458117,0.001658704],"category_scores_gemma":[0.0004807592,0.0004838232,0.0004887136,0.0008823129,0.0003577288,0.000516956,0.0006135251,0.001764988,0.001616663],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009437775,"about_ca_system_score_gemma":0.0006740825,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002188042,"about_ca_topic_score_gemma":0.00401657,"domain_scores_codex":[0.9996017,0.00003123251,0.00005039059,0.00009642676,0.0001267231,0.00009365524],"domain_scores_gemma":[0.9996361,0.00008392784,0.00003553911,0.0000985045,0.00007562809,0.00007033001],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008430997,0.00004417503,0.000140881,0.00008619056,0.000008720807,0.00005760401,0.00003298425,0.0001220849,0.9967635,0.0004424456,0.0002138261,0.002003204],"study_design_scores_gemma":[0.0000181492,0.00003283875,0.001544358,0.000006909043,0.00001480343,0.0001154899,0.00001574814,0.000917275,0.9882608,0.000159477,0.008904722,0.000009331458],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8410917,0.004784942,0.1281536,0.0008607259,0.0006997098,0.0005926055,0.01527373,0.001481043,0.007061981],"genre_scores_gemma":[0.7775642,0.005098595,0.1282624,0.0003207169,0.00009737253,0.0006335895,0.04066407,0.0006058849,0.04675312],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002188042,"threshold_uncertainty_score":0.00684762,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009574818329172707,"score_gpt":0.2540293732801535,"score_spread":0.2444545549509808,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}