{"id":"W2114458369","doi":"10.1186/1475-2859-12-97","title":"High-yield production of functional soluble single-domain antibodies in the cytoplasm of Escherichia coli","year":2013,"lang":"en","type":"article","venue":"Microbial Cell Factories","topic":"Monoclonal and Polyclonal Antibodies Research","field":"Medicine","cited_by":85,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"","keywords":"Escherichia coli; Yield (engineering); Cytoplasm; Antibody; Chemistry; Biology; Microbiology; Biochemistry; Computational biology; Immunology; Gene; Materials science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001802758,0.0001518583,0.0003352464,0.0001041654,0.00008079837,0.00001957435,0.0001221861,0.00008958584,0.0005084101],"category_scores_gemma":[0.00009071232,0.0001002747,0.00009771228,0.0002645788,0.0003402248,0.0001171937,0.00006090293,0.0002447361,0.00003170848],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000033069,"about_ca_system_score_gemma":0.0001000454,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001991525,"about_ca_topic_score_gemma":0.0002004371,"domain_scores_codex":[0.9987722,0.00007201044,0.0003648677,0.0002006774,0.0003311948,0.0002590178],"domain_scores_gemma":[0.999245,0.0001571143,0.0001179216,0.0002021197,0.0002354887,0.00004229766],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003491109,0.0003882291,0.02165495,0.000316753,0.0000311935,0.000003253281,0.001281194,0.00001490903,0.9622312,0.0002332094,0.0133599,0.0001360651],"study_design_scores_gemma":[0.0004209869,0.0004272361,0.09377646,0.00009107863,0.00002159122,0.00001378799,0.001179102,0.000003005989,0.8944589,0.000318638,0.009169193,0.0001199992],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9955779,0.0002443098,0.00002758273,0.001760824,0.0006203578,0.0004525181,0.00001781027,0.00001167989,0.001287011],"genre_scores_gemma":[0.9943172,0.00006974934,0.00067766,0.00008453726,0.0002523203,0.00001375201,0.00005106331,0.00001230603,0.004521355],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07212151,"threshold_uncertainty_score":0.5566734,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02956871334821113,"score_gpt":0.240968385396659,"score_spread":0.2113996720484478,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}