{"id":"W2114764466","doi":"10.1177/1087057108318783","title":"2-Tier Bacterial and In Vitro Selection of Active and Methotrexate-Resistant Variants of Human Dihydrofolate Reductase","year":2008,"lang":"en","type":"article","venue":"SLAS DISCOVERY","topic":"Biochemical and Molecular Research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":15,"is_retracted":false,"has_abstract":false,"ca_institutions":"Université de Montréal","funders":"Canadian Institutes of Health Research","keywords":"Dihydrofolate reductase; Active site; Protein-fragment complementation assay; Enzyme; In vitro; High-throughput screening; Mutant; Methotrexate; Biology; Biochemistry; Drug resistance; Computational biology; Complementation; Chemistry; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008587429,0.00009506396,0.0001682328,0.00005247763,0.00003429139,0.000006951788,0.00005450982,0.0001068212,0.000006187902],"category_scores_gemma":[0.00005113725,0.00008581873,0.0000384496,0.00008207208,0.0002031522,0.00001040353,0.000105661,0.00007641693,1.227515e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000007797078,"about_ca_system_score_gemma":0.00005029389,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001496294,"about_ca_topic_score_gemma":0.00002188049,"domain_scores_codex":[0.9992728,0.00007570143,0.0001690833,0.0002425341,0.00009371142,0.0001461986],"domain_scores_gemma":[0.9997305,0.000008786586,0.00006528383,0.0001147043,0.00003537217,0.00004536234],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.001523892,0.00008299548,0.0004338943,0.00003715135,0.00004367474,0.00000515186,0.00004742147,4.324556e-7,0.9974742,0.00003547537,0.00003300975,0.0002826884],"study_design_scores_gemma":[0.0006976138,0.0002242429,0.0112452,0.00001494233,0.00001104211,0.00001399863,0.00002244338,0.000007988669,0.9874768,0.0001371424,0.00005982511,0.0000887111],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9993376,0.0002202817,0.0000374989,0.00001663664,0.00002259726,0.0001101916,0.00006403323,0.000001582982,0.0001896445],"genre_scores_gemma":[0.9993337,0.0001940366,0.0001732856,0.000008933658,0.00002939754,0.000006055351,0.00007642061,0.00001021203,0.0001678974],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01081131,"threshold_uncertainty_score":0.3499586,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01255291323981236,"score_gpt":0.2705164014011012,"score_spread":0.2579634881612888,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}