{"id":"W2115475928","doi":"10.1139/o08-144","title":"Interconversion of a pair of active-site residues in <i>Escherichia coli</i> cystathionine γ-synthase, <i>E. coli</i> cystathionine β-lyase, and <i>Saccharomyces cerevisiae</i> cystathionine γ-lyase and development of tools for the investigation of their mechanisms and reaction specificity","year":2009,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cystathionine beta synthase; Transsulfuration; Cystathionine gamma-lyase; Mutant; Active site; Biochemistry; Escherichia coli; Saccharomyces cerevisiae; Enzyme kinetics; Lyase; Chemistry; Enzyme; Cysteine; Biology; Stereochemistry; Yeast; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002585612,0.0003795072,0.0001964687,0.0001549128,0.0001622855,0.0004381352,0.0003688362,0.0004162944,0.0004074537],"category_scores_gemma":[0.0003320327,0.0002054139,0.0003376247,0.0001798484,0.0003859613,0.000274034,0.0003050702,0.0006214203,0.0002916277],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005126277,"about_ca_system_score_gemma":0.0002634141,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007379938,"about_ca_topic_score_gemma":0.0007550512,"domain_scores_codex":[0.9997569,0.00006303048,0.00003695711,0.00006295028,0.00005089023,0.00002926709],"domain_scores_gemma":[0.9997639,0.00004559446,0.00008851099,0.00004272979,0.00001785125,0.00004138382],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003198547,0.00008492765,0.0006928144,0.00003414481,0.00001160938,0.000100677,0.00004323453,0.0002782247,0.9946737,0.000499348,0.00002908431,0.003232324],"study_design_scores_gemma":[0.00003487755,0.0002603398,0.002637279,0.000009630236,0.00002978573,0.0004794207,0.00004868189,0.001890113,0.9926202,0.00009369238,0.001883499,0.00001240613],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9939183,0.0002924187,0.004992911,0.00004459045,0.0000187985,0.00003435253,0.00009624055,0.00004474876,0.0005575639],"genre_scores_gemma":[0.9939965,0.0001856291,0.004767207,0.00004751913,0.000005736458,0.00002501246,0.0003144204,0.00002677301,0.0006312049],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007379938,"threshold_uncertainty_score":0.003719389,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01490216847406218,"score_gpt":0.2179953318570371,"score_spread":0.2030931633829749,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}