{"id":"W2115475928","doi":"10.1139/o08-144","title":"Interconversion of a pair of active-site residues in <i>Escherichia coli</i> cystathionine γ-synthase, <i>E. coli</i> cystathionine β-lyase, and <i>Saccharomyces cerevisiae</i> cystathionine γ-lyase and development of tools for the investigation of their mechanisms and reaction specificity","year":2009,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"Enzyme Structure and Function","field":"Materials Science","cited_by":22,"is_retracted":false,"has_abstract":true,"ca_institutions":"Carleton University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Cystathionine beta synthase; Transsulfuration; Cystathionine gamma-lyase; Mutant; Active site; Biochemistry; Escherichia coli; Saccharomyces cerevisiae; Enzyme kinetics; Lyase; Chemistry; Enzyme; Cysteine; Biology; Stereochemistry; Yeast; Gene","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0005789593,0.0002004157,0.000423169,0.00005897626,0.00009639675,0.00001351352,0.00008318362,0.0001895959,0.000008417021],"category_scores_gemma":[0.00007760042,0.0001474009,0.00003133803,0.0001312348,0.0003592747,0.0001455146,0.00006291171,0.0001026422,7.711541e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001875204,"about_ca_system_score_gemma":0.00007594479,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005894765,"about_ca_topic_score_gemma":0.00002039695,"domain_scores_codex":[0.9987333,0.00009253614,0.000567317,0.0003547157,0.00009346764,0.000158681],"domain_scores_gemma":[0.9987636,0.0003240151,0.0005444649,0.0001623806,0.0001436553,0.00006193366],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0009514023,0.00005491884,0.0004273605,0.0005181899,0.00001328939,4.802399e-7,0.0009867892,0.000001391461,0.9938266,0.0001823609,0.00003638125,0.0030008],"study_design_scores_gemma":[0.001066675,0.0005129639,0.004623138,0.0001794369,0.00003731223,0.000008876718,0.001438986,0.00004919248,0.9911392,0.000570289,0.0002389228,0.0001349822],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9969472,0.001311467,0.000877493,0.0001539929,0.00006015319,0.0003528872,0.0002373243,0.00001012288,0.0000493025],"genre_scores_gemma":[0.995191,0.0007497561,0.003831447,0.00006013953,0.00002453742,0.00001466565,0.0001041035,0.000006833179,0.00001745623],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004195778,"threshold_uncertainty_score":0.6010833,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01490216847406218,"score_gpt":0.2179953318570371,"score_spread":0.2030931633829749,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}