{"id":"W2116372927","doi":"10.1042/bj20030271","title":"Investigation of metal binding and activation of Escherichia coli glyoxalase I: kinetic, thermodynamic and mutagenesis studies","year":2004,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Advanced Glycation End Products research","field":"Biochemistry, Genetics and Molecular Biology","cited_by":63,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Waterloo","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Isothermal titration calorimetry; Chemistry; Metal; Metal ions in aqueous solution; Escherichia coli; Enzyme; Enzyme kinetics; Titration; Active site; Methylglyoxal; Biochemistry; Inorganic chemistry; Organic chemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002616432,0.0004954502,0.000417503,0.0001540819,0.0001422866,0.0003057774,0.0005683925,0.0003729926,0.0005475635],"category_scores_gemma":[0.0004794007,0.0001966812,0.0002445359,0.000286862,0.0001923551,0.0001708718,0.0002088273,0.0004317992,0.0002933256],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0006168075,"about_ca_system_score_gemma":0.0002250246,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002355963,"about_ca_topic_score_gemma":0.001805458,"domain_scores_codex":[0.9996579,0.00007889442,0.00002421219,0.00006245421,0.0001281417,0.00004850769],"domain_scores_gemma":[0.9997836,0.00005602834,0.00005398387,0.00001443504,0.00006682114,0.00002506478],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001263696,0.00004040734,0.0004442437,0.00005866329,0.00001095495,0.0000257308,0.00002156543,0.0004628024,0.9973218,0.00006747018,0.00002952824,0.001390508],"study_design_scores_gemma":[0.000004388925,0.00009356806,0.001711018,0.000002515497,0.00000863241,0.00009235686,0.0000178556,0.002101328,0.995275,0.00002041245,0.000666626,0.000006214271],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9925144,0.001252626,0.00478492,0.0001008467,0.00001058108,0.00003136145,0.0002856589,0.00006824801,0.0009514049],"genre_scores_gemma":[0.993668,0.0006327052,0.00401152,0.00003342098,0.000004081496,0.0000237911,0.0003624533,0.00003116123,0.001232944],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002355963,"threshold_uncertainty_score":0.004684448,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02798403518760494,"score_gpt":0.3035777119602258,"score_spread":0.2755936767726208,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}