{"id":"W2116585082","doi":"10.1139/o04-054","title":"Role of Serine 286 in cosubstrate binding and catalysis of a flavonol <i>O</i>-methyltransferase","year":2004,"lang":"en","type":"article","venue":"Biochemistry and Cell Biology","topic":"Plant Gene Expression Analysis","field":"Biochemistry, Genetics and Molecular Biology","cited_by":5,"is_retracted":false,"has_abstract":true,"ca_institutions":"Concordia University","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"O-methyltransferase; Stereochemistry; Chemistry; Methyltransferase; Biochemistry; Cofactor; Active site; Serine; Methylation; Homotetramer; Binding site; Pterin; Isothermal titration calorimetry; Circular dichroism; Tetranitromethane; Enzyme; Protein subunit; DNA","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":true,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00009962451,0.0002886378,0.0002876143,0.00007793343,0.0001982687,0.0003236786,0.0002333358,0.0004292271,0.001227348],"category_scores_gemma":[0.0002110287,0.0001251708,0.0002335913,0.00006799241,0.0001955076,0.0002207778,0.0002259597,0.0003517849,0.001038117],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000288895,"about_ca_system_score_gemma":0.0002134274,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005735139,"about_ca_topic_score_gemma":0.0004516692,"domain_scores_codex":[0.9998959,0.00001672195,0.000007873118,0.00003254596,0.00002999999,0.00001682797],"domain_scores_gemma":[0.9998257,0.00003012592,0.00005723648,0.00001208047,0.00002110864,0.00005385954],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0003158715,0.00002969343,0.0007468197,0.00005637059,0.000005201575,0.0002687442,0.00003100151,0.0002622935,0.9964252,0.0001936817,0.00005424406,0.001610905],"study_design_scores_gemma":[0.00004093173,0.0002006288,0.01591477,0.000008319711,0.00001309617,0.0008363848,0.00007979823,0.003416698,0.9768512,0.0001784947,0.002444278,0.00001550597],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9952286,0.0007427233,0.002475826,0.0001168261,0.00002666441,0.000008327527,0.0001208617,0.0000744924,0.001205589],"genre_scores_gemma":[0.9963569,0.0003022037,0.001439827,0.00003567677,0.000007714223,0.000003995245,0.0003189399,0.00001480454,0.001519831],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001227348,"threshold_uncertainty_score":0.004105866,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.003557662121292904,"score_gpt":0.2075378137821255,"score_spread":0.2039801516608326,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}