{"id":"W2117216932","doi":"10.1128/jb.182.2.498-503.2000","title":"The N-Terminal Region of the <i>Escherichia coli</i> WecA (Rfe) Protein, Containing Three Predicted Transmembrane Helices, Is Required for Function but Not for Membrane Insertion","year":2000,"lang":"en","type":"article","venue":"Journal of Bacteriology","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":37,"is_retracted":false,"has_abstract":true,"ca_institutions":"Western University","funders":"","keywords":"Biology; Escherichia coli; Transmembrane protein; Escherichia coli Proteins; Transmembrane domain; Membrane protein; Terminal (telecommunication); Function (biology); Membrane; Bacterial protein; Membrane transport protein; Biophysics; Transport protein; Biochemistry; Cell biology; Bacteria; Genetics; Gene","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001806884,0.0004655453,0.0002893986,0.000166224,0.0002614031,0.0004603417,0.000315979,0.0006328944,0.0008232634],"category_scores_gemma":[0.0004632291,0.0003199922,0.0003574651,0.0001639028,0.0005750144,0.0003524287,0.0003116353,0.0007779815,0.0009193912],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005233348,"about_ca_system_score_gemma":0.0004983679,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001493163,"about_ca_topic_score_gemma":0.001791555,"domain_scores_codex":[0.9997875,0.00002944209,0.00002580259,0.00004922583,0.00005413979,0.00005398017],"domain_scores_gemma":[0.9993913,0.0001613956,0.0001684704,0.00008484862,0.00006201385,0.000131935],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004359993,0.00001230201,0.0001521252,0.00001771533,0.000002618015,0.00005003679,0.000009533664,0.00003461854,0.9992411,0.00007030806,0.0000238861,0.0003420624],"study_design_scores_gemma":[0.00001840202,0.0000606768,0.006307209,0.00001294427,0.0000126591,0.0007098971,0.00003267651,0.0005920643,0.9900247,0.00006022592,0.00215706,0.00001152982],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9921084,0.0005280418,0.005492722,0.0001898459,0.00003107158,0.0000257095,0.0006056706,0.0001002044,0.0009182745],"genre_scores_gemma":[0.9899693,0.000228825,0.005802037,0.0001187628,0.00001210725,0.00002523471,0.002253994,0.00007212166,0.001517489],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001493163,"threshold_uncertainty_score":0.003797054,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01400924429233067,"score_gpt":0.2243558197205737,"score_spread":0.2103465754282431,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}