{"id":"W2118726157","doi":"10.1042/bj20060874","title":"Directed evolution of a monomeric, bright and photostable version of <i>Clavularia</i> cyan fluorescent protein: structural characterization and applications in fluorescence imaging","year":2006,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":401,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Basic Energy Sciences; National Center for Research Resources; Natural Sciences and Engineering Research Council of Canada; University of California, San Diego; Office of Science; National Institutes of Health; University of Alberta; U.S. Department of Energy","keywords":"Aequorea victoria; Förster resonance energy transfer; Cyan; Fluorescence; Green fluorescent protein; Chromophore; Protein engineering; Protein tag; Dimer; Monomer; Biophysics; Yellow fluorescent protein; Biology; Chemistry; Biochemistry; Photochemistry; Gene; Recombinant DNA; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008316325,0.0001189831,0.000154137,0.00007840611,0.00005364606,0.00001453468,0.00009519079,0.00008564518,0.000002406501],"category_scores_gemma":[0.00002418414,0.0001111991,0.00002968309,0.0001373921,0.0001854829,0.00001925792,0.00007761763,0.000109799,6.35568e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004548921,"about_ca_system_score_gemma":0.00004285846,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00005767896,"about_ca_topic_score_gemma":0.000001637222,"domain_scores_codex":[0.9991821,0.00003057296,0.0003120948,0.00021565,0.0001019947,0.0001576013],"domain_scores_gemma":[0.9994927,0.000004842049,0.0002187164,0.0001221907,0.0001121992,0.00004933537],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008999499,0.0000362809,0.03615038,0.00003431117,0.000003834793,5.834633e-7,0.000009134103,0.000001256655,0.9626672,0.00003876411,0.00002045208,0.0009477839],"study_design_scores_gemma":[0.0003633513,0.00004005292,0.04892864,0.00008961843,0.000007552937,0.00002751087,0.00001739504,0.0002566989,0.9498284,0.0001857757,0.0001495072,0.0001054263],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9780324,0.001066012,0.02051935,0.00002284321,0.00001286185,0.0002978327,0.0000306256,0.000009914659,0.000008163258],"genre_scores_gemma":[0.9872739,0.0001466415,0.01238201,0.000004685813,0.00005541291,0.00001262583,0.0001062923,0.00001112493,0.000007272163],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01283875,"threshold_uncertainty_score":0.4534569,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.001722302979563333,"score_gpt":0.208728337336695,"score_spread":0.2070060343571317,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}