{"id":"W2118726157","doi":"10.1042/bj20060874","title":"Directed evolution of a monomeric, bright and photostable version of <i>Clavularia</i> cyan fluorescent protein: structural characterization and applications in fluorescence imaging","year":2006,"lang":"en","type":"article","venue":"Biochemical Journal","topic":"Advanced Fluorescence Microscopy Techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":401,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Basic Energy Sciences; National Center for Research Resources; Natural Sciences and Engineering Research Council of Canada; University of California, San Diego; Office of Science; National Institutes of Health; University of Alberta; U.S. Department of Energy","keywords":"Aequorea victoria; Förster resonance energy transfer; Cyan; Fluorescence; Green fluorescent protein; Chromophore; Protein engineering; Protein tag; Dimer; Monomer; Biophysics; Yellow fluorescent protein; Biology; Chemistry; Biochemistry; Photochemistry; Gene; Recombinant DNA; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001932887,0.0003321287,0.0002131099,0.000181635,0.0001475519,0.0003323903,0.0003814304,0.0002967686,0.0003523646],"category_scores_gemma":[0.0003028276,0.0001509268,0.0002433553,0.0002773561,0.0002071984,0.0002041665,0.0002370376,0.0006390029,0.0002079331],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00048943,"about_ca_system_score_gemma":0.0003475488,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001666163,"about_ca_topic_score_gemma":0.001323546,"domain_scores_codex":[0.9998996,0.00001108025,0.00001198078,0.00002599493,0.00003408306,0.00001721221],"domain_scores_gemma":[0.9998543,0.00001941669,0.00005714295,0.00001939373,0.00002095744,0.00002871545],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001699647,0.000009815369,0.0002350743,0.00001865449,0.000002984357,0.00002706461,0.00001614095,0.0002384837,0.9978641,0.00009148219,0.0000153953,0.001463841],"study_design_scores_gemma":[0.000004454001,0.00005435188,0.001767441,0.000003201525,0.00001053813,0.0001955988,0.00001521097,0.001563405,0.9949839,0.00002427151,0.001371826,0.0000056546],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9823238,0.0003043066,0.01622588,0.00007510333,0.00001067237,0.00005480787,0.0003698817,0.00009077932,0.0005448874],"genre_scores_gemma":[0.9638333,0.0006643323,0.03240652,0.00004449204,0.000005400994,0.00005612635,0.00138778,0.0001026629,0.001499452],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001666163,"threshold_uncertainty_score":0.003551066,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.001722302979563333,"score_gpt":0.208728337336695,"score_spread":0.2070060343571317,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}