{"id":"W2119289645","doi":"10.1016/j.pep.2008.03.013","title":"Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design","year":2008,"lang":"en","type":"article","venue":"Protein Expression and Purification","topic":"Insect Resistance and Genetics","field":"Biochemistry, Genetics and Molecular Biology","cited_by":27,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Canadian Institutes of Health Research; Ottawa Hospital Research Institute","keywords":"Proprotein convertase; Proprotein Convertases; Subtilisin; Biochemistry; Proteolysis; Kexin; Recombinant DNA; Biology; Enzyme; Proteinase K; Peptide; Enteropeptidase; Protein precursor; Chemistry; Molecular biology; Fusion protein; Lipoprotein","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003434674,0.0006940425,0.0004929512,0.0005032766,0.0003847855,0.000441747,0.0007188473,0.0003585499,0.001279886],"category_scores_gemma":[0.0003598917,0.00039803,0.0004368323,0.0007608776,0.0003039907,0.000344222,0.000242288,0.001038127,0.001009205],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0008366928,"about_ca_system_score_gemma":0.0007787273,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.003690333,"about_ca_topic_score_gemma":0.003414521,"domain_scores_codex":[0.9997702,0.00003708916,0.00002963284,0.00003990974,0.0000776121,0.00004550878],"domain_scores_gemma":[0.9998288,0.00003647227,0.00002355251,0.00002547911,0.00005732543,0.00002822115],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001895478,0.00003656241,0.0001687827,0.000146393,0.00001470655,0.00007186508,0.00001644013,0.0001516222,0.9963028,0.0001769265,0.0001848866,0.00253936],"study_design_scores_gemma":[0.00001937478,0.00005300023,0.0005331759,0.000003924404,0.00002410822,0.0001768097,0.000008277002,0.0004776585,0.9921306,0.00003565176,0.006531773,0.000005589263],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8813961,0.00574561,0.09581523,0.001075667,0.0001950248,0.001148624,0.008997078,0.0008888346,0.004737828],"genre_scores_gemma":[0.7828407,0.005480323,0.1470207,0.0002492964,0.00006081392,0.0006495527,0.04158861,0.0003023889,0.02180769],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.003690333,"threshold_uncertainty_score":0.007337689,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01698891999334998,"score_gpt":0.2272919253616462,"score_spread":0.2103030053682962,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}