{"id":"W2121302417","doi":"10.1128/aem.68.7.3261-3269.2002","title":"Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from<i>Bacillus subtilis</i>: Effects of Molecular Chaperones and a Wall-Bound Protease on Antibody Fragment Production","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":200,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada","keywords":"Bacillus subtilis; Protease; Monoclonal antibody; Antibody; Biochemistry; Biology; Extracellular; Chaperone (clinical); Chemistry; Molecular biology; Bacteria; Enzyme; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0000919714,0.0001868841,0.000205357,0.00006017468,0.00007526227,0.000007786112,0.00003979298,0.0001406835,0.00002381926],"category_scores_gemma":[0.00001199417,0.0001786559,0.00002881706,0.00004561741,0.0003762817,0.000008432355,0.000068992,0.0000677842,0.000002244376],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001659136,"about_ca_system_score_gemma":0.000003491464,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.000004959994,"about_ca_topic_score_gemma":7.633574e-7,"domain_scores_codex":[0.9988844,0.00006752562,0.000251188,0.0005893371,0.00006402403,0.0001435312],"domain_scores_gemma":[0.999544,0.000009684336,0.0001572404,0.00023016,0.000009860671,0.00004908386],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001960408,0.0004420169,0.002229641,0.00009225041,0.00004498713,5.486418e-7,0.0001389505,0.000004926839,0.9928216,0.00004211994,0.000007204763,0.003979682],"study_design_scores_gemma":[0.0005321231,0.0004369456,0.04623545,0.00002641369,0.00001929224,0.00001655828,0.00004572223,0.00000966143,0.9510084,0.00003345744,0.001479435,0.0001565644],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.996497,0.001732555,0.0004346204,0.000209496,0.00009381218,0.0009433526,0.00006868804,0.000007078854,0.00001342143],"genre_scores_gemma":[0.9961925,0.00232721,0.0001288213,0.00005992416,0.00005472182,0.00006559107,0.001119036,0.00001377891,0.000038435],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.04400581,"threshold_uncertainty_score":0.7285376,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006196560324513926,"score_gpt":0.1774668703590053,"score_spread":0.1712703100344914,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}