{"id":"W2121302417","doi":"10.1128/aem.68.7.3261-3269.2002","title":"Functional Production and Characterization of a Fibrin-Specific Single-Chain Antibody Fragment from<i>Bacillus subtilis</i>: Effects of Molecular Chaperones and a Wall-Bound Protease on Antibody Fragment Production","year":2002,"lang":"en","type":"article","venue":"Applied and Environmental Microbiology","topic":"Protein purification and stability","field":"Biochemistry, Genetics and Molecular Biology","cited_by":200,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Calgary","funders":"Natural Sciences and Engineering Research Council of Canada; Heart and Stroke Foundation of Canada","keywords":"Bacillus subtilis; Protease; Monoclonal antibody; Antibody; Biochemistry; Biology; Extracellular; Chaperone (clinical); Chemistry; Molecular biology; Bacteria; Enzyme; Immunology","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002036614,0.0003140344,0.0002396944,0.0001422014,0.0001066704,0.0002404358,0.0002028334,0.0002762951,0.0003557019],"category_scores_gemma":[0.0002607181,0.0001039675,0.0002396981,0.0001858141,0.0001244116,0.0001422793,0.0001057879,0.0003286212,0.0003115762],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002085987,"about_ca_system_score_gemma":0.0001698881,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.001154277,"about_ca_topic_score_gemma":0.0006054842,"domain_scores_codex":[0.9998763,0.00002308084,0.00001728072,0.00002128814,0.00004028246,0.00002173051],"domain_scores_gemma":[0.999823,0.0000308396,0.0000522783,0.00001712849,0.00003691764,0.00003969881],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001560501,0.00001116258,0.00007833798,0.00001351622,0.000001259412,0.00001835583,0.000005931993,0.00003098071,0.9993978,0.00001081725,0.000007398512,0.0004089277],"study_design_scores_gemma":[0.00000528908,0.0001183658,0.001932087,0.000002904823,0.000006839411,0.0001732901,0.00001009686,0.0004014977,0.9966506,0.000008174228,0.0006877826,0.000002939328],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9912642,0.0005232928,0.006897565,0.00007688306,0.00001272436,0.00003148301,0.0005364227,0.00005092901,0.0006065422],"genre_scores_gemma":[0.9827101,0.0004720045,0.01284056,0.00004300485,0.000007824821,0.00003120097,0.00267073,0.00004916257,0.001175474],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001154277,"threshold_uncertainty_score":0.002295196,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.006196560324513926,"score_gpt":0.1774668703590053,"score_spread":0.1712703100344914,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}