{"id":"W2122184569","doi":"10.1111/j.1755-0998.2009.02825.x","title":"Rapid and reliable high‐throughput methods of DNA extraction for use in barcoding and molecular systematics of mushrooms","year":2009,"lang":"en","type":"article","venue":"Molecular Ecology Resources","topic":"Mycorrhizal Fungi and Plant Interactions","field":"Agricultural and Biological Sciences","cited_by":148,"is_retracted":false,"has_abstract":true,"ca_institutions":"Royal Ontario Museum; University of Toronto","funders":"Ontario Genomics Institute; Genome Canada","keywords":"Biology; DNA barcoding; Systematics; DNA extraction; Evolutionary biology; Extraction (chemistry); Computational biology; Zoology; Genetics; Taxonomy (biology); Gene; Polymerase chain reaction; Chromatography","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.003118864,0.001248795,0.0009197561,0.002443328,0.001245914,0.001754168,0.001623457,0.00137171,0.002954603],"category_scores_gemma":[0.006436745,0.000904923,0.0009885447,0.001035892,0.001263509,0.001809963,0.001862119,0.002288747,0.004710536],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0003889596,"about_ca_system_score_gemma":0.00108254,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0005337775,"about_ca_topic_score_gemma":0.001736357,"domain_scores_codex":[0.9959422,0.001010845,0.000532605,0.0005832257,0.001724168,0.0002069517],"domain_scores_gemma":[0.9948609,0.001520375,0.001088652,0.001228411,0.001051775,0.0002497867],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001133108,0.0001214609,0.001977586,0.0003943474,0.00003889019,0.0002266705,0.0002163786,0.00047701,0.9390159,0.0008577165,0.00114655,0.05541434],"study_design_scores_gemma":[0.00007417976,0.0005397437,0.01086747,0.0001956251,0.0001007095,0.001499608,0.0002694792,0.005275479,0.9512047,0.002129643,0.02767779,0.0001656481],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.05922088,0.002567633,0.9293591,0.001021306,0.0003454832,0.001764401,0.002005455,0.001516837,0.002198834],"genre_scores_gemma":[0.1085554,0.002578008,0.8785502,0.0004038668,0.0002338525,0.002418865,0.003914644,0.0002825762,0.003062571],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.003118864,"threshold_uncertainty_score":0.01649427,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01817013800968111,"score_gpt":0.2647941663671153,"score_spread":0.2466240283574342,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}