{"id":"W2123022248","doi":"10.1111/j.1574-6968.2000.tb08972.x","title":"Cloning, over-expression and purification of<i>Pseudomonas aeruginosa murC</i>encoding uridine diphosphate<i>N</i>-acetylmuramate: L-alanine ligase","year":2000,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"Bacterial Genetics and Biotechnology","field":"Biochemistry, Genetics and Molecular Biology","cited_by":13,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université Laval","funders":"Global Health and Health Policy, Harvard University","keywords":"DNA ligase; Cloning (programming); Uridine diphosphate; Chemistry; Biochemistry; Uridine; Alanine; Molecular cloning; Pseudomonas aeruginosa; Molecular biology; Biology; Gene; Enzyme; Bacteria; Gene expression; Genetics; RNA; Amino acid; Computer science","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.000189134,0.0003667722,0.0004691212,0.0002043288,0.0002351351,0.0003678707,0.0002792029,0.000257746,0.0008753113],"category_scores_gemma":[0.000203913,0.0001660021,0.0003086328,0.0003906374,0.0001986617,0.0002078316,0.0003183171,0.0005532346,0.001014666],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005356608,"about_ca_system_score_gemma":0.0004097855,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.002372144,"about_ca_topic_score_gemma":0.002614005,"domain_scores_codex":[0.9998322,0.00002165053,0.00001574629,0.00003115337,0.00005101361,0.00004822686],"domain_scores_gemma":[0.9998666,0.0000174415,0.00002544176,0.0000204784,0.00003156004,0.00003850273],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005920417,0.00002625691,0.0002374963,0.0001075064,0.000003475363,0.0001182662,0.00003352013,0.0000778821,0.9956801,0.0002346025,0.0001597667,0.003261959],"study_design_scores_gemma":[0.00001574353,0.000140625,0.002965031,0.00001147762,0.00001367348,0.0004385826,0.00004727058,0.000813383,0.9766105,0.0001006711,0.01883156,0.00001151827],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9427723,0.004120682,0.04124052,0.0007736612,0.0001291569,0.0004156843,0.005217836,0.0006292587,0.004700871],"genre_scores_gemma":[0.9065404,0.003273098,0.06164857,0.0002145737,0.00004335522,0.0002568531,0.01298612,0.0002060522,0.01483105],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002372144,"threshold_uncertainty_score":0.004716694,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004636271855685902,"score_gpt":0.2015172627894574,"score_spread":0.1968809909337715,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}