{"id":"W2123737549","doi":"10.1093/protein/14.12.983","title":"Kinetic characterization of ribonuclease S mutants containing photoisomerizable phenylazophenylalanine residues","year":2001,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Photochromic and Fluorescence Chemistry","field":"Materials Science","cited_by":50,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"Natural Sciences and Engineering Research Council of Canada","keywords":"Photoisomerization; Chemistry; Ribonuclease; Enzyme; Stereochemistry; RNase P; Kinetics; Azobenzene; Kinetic energy; Crystallography; Reaction rate constant; Substrate (aquarium); Isomerization; Biochemistry; Molecule; Biology; Organic chemistry; Catalysis; RNA; Physics","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002010687,0.0001326044,0.0001755064,0.00006299587,0.00006336137,0.00003473303,0.00007123567,0.00006887411,0.0001292685],"category_scores_gemma":[0.00006853054,0.0001350148,0.00001832459,0.0001943321,0.00003180793,0.0001724654,0.00001676734,0.00007775537,0.000005034991],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000323207,"about_ca_system_score_gemma":0.00002496,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00003674983,"about_ca_topic_score_gemma":3.774411e-7,"domain_scores_codex":[0.9992135,0.00002535209,0.0002160018,0.0002144171,0.000122047,0.0002086496],"domain_scores_gemma":[0.9996944,0.00001848797,0.00009098128,0.00007666311,0.00005315748,0.00006630701],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001460892,0.00002596075,0.0001654036,0.00006737456,0.000004792205,0.000003281079,0.0001045373,0.0006162318,0.998543,0.00003004054,0.000002047714,0.0002912282],"study_design_scores_gemma":[0.000326296,0.0001314502,0.001465212,0.0001099075,0.000009115402,0.00003053653,0.0000104708,0.05254633,0.9451366,0.00002464489,0.0000713941,0.0001380238],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8558652,0.00004939041,0.1435727,0.000009458307,0.00003819861,0.0003249021,0.00000291123,0.0001091632,0.00002810198],"genre_scores_gemma":[0.9934728,0.00003572661,0.00622033,0.000008430794,0.00006458173,0.00006815795,0.000007658511,0.0000201201,0.0001021883],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1376076,"threshold_uncertainty_score":0.5505744,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.009260626519482654,"score_gpt":0.1926649569647412,"score_spread":0.1834043304452586,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}