{"id":"W2123815804","doi":"10.1002/pmic.200600518","title":"Comparison of SDS‐ and methanol‐assisted protein solubilization and digestion methods for <b><i>Escherichia coli</i></b> membrane proteome analysis by 2‐D LC‐MS/MS","year":2007,"lang":"en","type":"article","venue":"PROTEOMICS","topic":"Advanced Proteomics Techniques and Applications","field":"Chemistry","cited_by":120,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Alberta","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Solubilization; Proteome; Escherichia coli; Chromatography; Chemistry; Digestion (alchemy); Membrane protein; Proteomics; Methanol; Membrane; Biochemistry","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["metaepi_narrow"],"consensus_categories":[],"category_scores_codex":[0.001065497,0.0003028535,0.0006615759,0.0001648083,0.0002228736,0.00005305908,0.0001962011,0.0003330365,0.00002260361],"category_scores_gemma":[0.0002516165,0.0003132313,0.0001240225,0.000641254,0.0001868854,0.0001577393,0.0001024387,0.0002748551,2.933126e-7],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0000881185,"about_ca_system_score_gemma":0.00003967137,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00007758522,"about_ca_topic_score_gemma":0.0000285412,"domain_scores_codex":[0.9980288,0.00005682334,0.0007961135,0.0005879962,0.0001698234,0.0003604782],"domain_scores_gemma":[0.9983011,0.0001973465,0.0006856906,0.0004270796,0.0002387189,0.0001500808],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001883498,0.0001663739,0.0020446,0.0004499819,0.0001491043,1.071057e-7,0.0001624696,0.00004843827,0.986374,0.0009095329,0.000009175114,0.00949785],"study_design_scores_gemma":[0.0006157797,0.00009477759,0.000341281,0.00005410394,0.0003291883,0.000001463934,0.00006847311,0.01431043,0.9792522,0.003090945,0.001507786,0.0003335411],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"methods","genre_gemma":"methods","genre_scores_codex":[0.3903067,0.0002385829,0.6070698,0.00009855889,0.00000634648,0.001934626,0.00006787007,0.0001047582,0.0001727098],"genre_scores_gemma":[0.3516769,0.00005547445,0.646422,0.00001554519,0.00002788969,0.001413148,0.0001676139,0.00004170459,0.00017974],"genre_candidate":"methods","genre_consensus":"methods","teacher_disagreement_score":0.03935216,"threshold_uncertainty_score":0.999932,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02701426125309679,"score_gpt":0.371495558090171,"score_spread":0.3444812968370742,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}