{"id":"W2123960848","doi":"10.1002/anie.201408015","title":"Coumarin‐Based Fluorogenic Probes for No‐Wash Protein Labeling","year":2014,"lang":"en","type":"article","venue":"Angewandte Chemie International Edition","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":91,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Canadian Institutes of Health Research","keywords":"Maleimide; Fluorophore; Chemistry; Reactivity (psychology); Fluorescence; Peptide; Thiol; Reagent; Dithiol; Intracellular; Combinatorial chemistry; Glutathione; Bioorthogonal chemistry; Cysteine; Coumarin; Substituent; Biochemistry; Stereochemistry; Organic chemistry; Click chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":["insufficient_payload"],"consensus_categories":[],"category_scores_codex":[0.0001406366,0.0001719857,0.0001340414,0.00003019914,0.0001388678,0.00006745847,0.0002930183,0.0001461121,0.001608064],"category_scores_gemma":[0.0003587211,0.0001855326,0.000136561,0.00005925714,0.00005455936,0.0001307839,0.00004074655,0.0001456852,0.00007999955],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0001218887,"about_ca_system_score_gemma":0.00004655915,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00001105496,"about_ca_topic_score_gemma":0.000003952883,"domain_scores_codex":[0.9988893,0.000004069943,0.0002817072,0.0003533227,0.0002686625,0.0002029398],"domain_scores_gemma":[0.9989743,0.0001434105,0.0001613822,0.0002468051,0.0003945536,0.00007960002],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00005220701,0.0001406182,0.00008326329,0.0001480834,0.00003850391,3.073297e-7,0.00001434937,0.00002381585,0.9884313,0.0008165684,0.009217051,0.001033876],"study_design_scores_gemma":[0.0008084508,0.00001186468,0.000008677482,0.0001092378,0.00002102352,0.000002588547,0.00001196714,0.002034632,0.8307765,0.001692401,0.1643455,0.0001772251],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6815754,0.000119605,0.08116876,0.007997662,0.001323977,0.00120447,0.001723571,0.001082875,0.2238037],"genre_scores_gemma":[0.9813036,0.000006002785,0.003374786,0.000289742,0.003569631,0.001249292,0.003550853,0.00003548957,0.006620644],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2997282,"threshold_uncertainty_score":0.9993046,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01533698333748145,"score_gpt":0.2612675980380541,"score_spread":0.2459306147005726,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}