{"id":"W2123960848","doi":"10.1002/anie.201408015","title":"Coumarin‐Based Fluorogenic Probes for No‐Wash Protein Labeling","year":2014,"lang":"en","type":"article","venue":"Angewandte Chemie International Edition","topic":"Click Chemistry and Applications","field":"Chemistry","cited_by":91,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Ottawa","funders":"Canadian Institutes of Health Research","keywords":"Maleimide; Fluorophore; Chemistry; Reactivity (psychology); Fluorescence; Peptide; Thiol; Reagent; Dithiol; Intracellular; Combinatorial chemistry; Glutathione; Bioorthogonal chemistry; Cysteine; Coumarin; Substituent; Biochemistry; Stereochemistry; Organic chemistry; Click chemistry; Enzyme","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0004842948,0.0005684304,0.0003561116,0.0003711341,0.0003187633,0.0003486377,0.000594593,0.0005715567,0.001173724],"category_scores_gemma":[0.0003972976,0.0002309361,0.0002182654,0.0004497221,0.0003591414,0.0004674134,0.0002834009,0.0009793866,0.0006502083],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0009825227,"about_ca_system_score_gemma":0.0003397975,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0006385725,"about_ca_topic_score_gemma":0.001623379,"domain_scores_codex":[0.999663,0.00005890506,0.00001285464,0.00007414933,0.0001160194,0.00007502503],"domain_scores_gemma":[0.9997992,0.0000530814,0.00004121831,0.0000342907,0.00004186573,0.00003043805],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00004561643,0.0000207584,0.00005322264,0.0000569826,0.000003302226,0.00003729267,0.00001810284,0.0001840872,0.9960185,0.001076997,0.0001660465,0.002319126],"study_design_scores_gemma":[0.000003941411,0.00003296668,0.0001854925,0.000002366479,0.000003079705,0.00006123249,0.000003557286,0.0008455769,0.9939871,0.00006919646,0.004801377,0.00000418433],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6341193,0.00676618,0.3463366,0.000787966,0.0002525279,0.0003698227,0.0009269772,0.001232309,0.009208284],"genre_scores_gemma":[0.9040371,0.004913223,0.07665821,0.0001871315,0.00005303,0.0003219512,0.001120983,0.0001159595,0.01259241],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.001173724,"threshold_uncertainty_score":0.007128716,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01533698333748145,"score_gpt":0.2612675980380541,"score_spread":0.2459306147005726,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}