{"id":"W2124247856","doi":"10.1111/j.1574-6968.2000.tb09224.x","title":"Ribonuclease U2: cloning, production in<i>Pichia pastoris</i>and affinity chromatography purification of the active recombinant protein","year":2000,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Pichia pastoris; Recombinant DNA; Ribonuclease; Affinity chromatography; RNase P; Biochemistry; Pichia; Complementary DNA; Endoribonuclease; Amino acid; Biology; Yeast; Signal peptide; Molecular biology; FLAG-tag; Enzyme; RNA; Gene; Fusion protein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0003900691,0.0009453843,0.0007262892,0.000445736,0.000311813,0.000606638,0.000401428,0.0003764451,0.0008974258],"category_scores_gemma":[0.0002707542,0.0003292186,0.0004302918,0.0009664618,0.0002730427,0.0002953849,0.0004309786,0.0007298886,0.001272104],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000460422,"about_ca_system_score_gemma":0.0003084618,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0009631009,"about_ca_topic_score_gemma":0.00101232,"domain_scores_codex":[0.9997177,0.00005292382,0.0000280856,0.00006327294,0.00007836193,0.00005964117],"domain_scores_gemma":[0.999898,0.00001925383,0.0000191554,0.00001956752,0.00001652653,0.00002749635],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001349887,0.00004664044,0.0002258825,0.0001358243,0.00001301794,0.0001141012,0.00003271775,0.0001824536,0.9943,0.0002043764,0.0003324076,0.004277559],"study_design_scores_gemma":[0.00004131142,0.0001550335,0.001971784,0.00001814419,0.00002647104,0.0003620205,0.00002031796,0.001061844,0.9804958,0.0001037617,0.01572907,0.00001459162],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8745387,0.01214446,0.09025518,0.0008978824,0.000331124,0.0007946689,0.01038827,0.002101716,0.008548063],"genre_scores_gemma":[0.8325596,0.01007127,0.1004092,0.000360403,0.0001552568,0.000691128,0.03147777,0.0005000042,0.02377537],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0009631009,"threshold_uncertainty_score":0.003340542,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005415136275009444,"score_gpt":0.1843633670282574,"score_spread":0.1789482307532479,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}