{"id":"W2124247856","doi":"10.1111/j.1574-6968.2000.tb09224.x","title":"Ribonuclease U2: cloning, production in<i>Pichia pastoris</i>and affinity chromatography purification of the active recombinant protein","year":2000,"lang":"en","type":"article","venue":"FEMS Microbiology Letters","topic":"Toxin Mechanisms and Immunotoxins","field":"Immunology and Microbiology","cited_by":9,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"","keywords":"Pichia pastoris; Recombinant DNA; Ribonuclease; Affinity chromatography; RNase P; Biochemistry; Pichia; Complementary DNA; Endoribonuclease; Amino acid; Biology; Yeast; Signal peptide; Molecular biology; FLAG-tag; Enzyme; RNA; Gene; Fusion protein","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002871198,0.0002318285,0.0003219473,0.0001449428,0.0002346899,0.000008563229,0.0002859816,0.0002968475,0.000259646],"category_scores_gemma":[0.00004666223,0.000178352,0.0001170348,0.0002817656,0.0005598235,0.0001076858,0.00006551192,0.0005031138,0.00006788842],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00004920323,"about_ca_system_score_gemma":0.00003377251,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0002578215,"about_ca_topic_score_gemma":0.00007320916,"domain_scores_codex":[0.998273,0.000455034,0.0004053673,0.000466971,0.00001962826,0.0003800518],"domain_scores_gemma":[0.9992135,0.00003243543,0.000249973,0.0004571266,0.00003143821,0.00001555602],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0002094838,0.0001789391,0.001784302,0.00001499992,0.00007519292,0.000001043823,0.0007526866,0.000004691601,0.9908708,0.0001050319,0.001234019,0.004768777],"study_design_scores_gemma":[0.001042388,0.0001499806,0.06149723,0.0001009757,0.00002983411,0.00008666272,0.0001130703,4.195478e-7,0.9131308,0.00009711103,0.02352658,0.0002249054],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9949427,0.0005240223,0.000006871663,0.002446651,0.001103251,0.0008263385,0.00003968303,0.00004219069,0.00006830054],"genre_scores_gemma":[0.998661,0.00008111581,0.0001596274,0.0004043398,0.00002887888,0.00007795585,0.00006664579,0.0000204279,0.0004999658],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.07773999,"threshold_uncertainty_score":0.7272982,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005415136275009444,"score_gpt":0.1843633670282574,"score_spread":0.1789482307532479,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}