{"id":"W2125546186","doi":"10.1016/j.ymben.2005.06.002","title":"Chemostat culture characterization of Escherichia coli mutant strains metabolically engineered for aerobic succinate production: A study of the modified metabolic network based on metabolite profile, enzyme activity, and gene expression profile","year":2005,"lang":"en","type":"article","venue":"Metabolic Engineering","topic":"Microbial Metabolic Engineering and Bioproduction","field":"Biochemistry, Genetics and Molecular Biology","cited_by":43,"is_retracted":false,"has_abstract":false,"ca_institutions":"","funders":"Andlinger Center for Energy and the Environment, Princeton University; Canadian Association for the Study of the Liver; National Science Foundation","keywords":"Malate synthase; Mutant; Escherichia coli; Biochemistry; Chemostat; Isocitrate lyase; Malate dehydrogenase; Strain (injury); Enzyme; Succinate dehydrogenase; Biology; Gene; Molecular biology; Chemistry; Glyoxylate cycle; Bacteria; Genetics","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001232463,0.0003828218,0.0002896379,0.000275043,0.000132282,0.0002454074,0.0002237592,0.0001743276,0.00058207],"category_scores_gemma":[0.000219797,0.0001708003,0.0002145886,0.0003942098,0.0001295658,0.0001864356,0.0001768372,0.0003307097,0.0002470489],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002855449,"about_ca_system_score_gemma":0.000386855,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.004788325,"about_ca_topic_score_gemma":0.005006038,"domain_scores_codex":[0.9998341,0.00001389813,0.00002822736,0.00003747461,0.00006062934,0.00002556594],"domain_scores_gemma":[0.9998131,0.00003956598,0.00003949397,0.00002275687,0.00005267393,0.00003246289],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007020035,0.00002404291,0.0006032698,0.000007983053,0.000002619926,0.0000182621,0.000009158318,0.00003921419,0.998665,0.00001888456,0.00001765394,0.0005236218],"study_design_scores_gemma":[0.000004482175,0.0001172408,0.01256256,0.000002003188,0.00001442592,0.00007953486,0.00002678968,0.0008400087,0.9860043,0.00001483888,0.0003296254,0.000004263132],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9927818,0.0003032383,0.003491235,0.00008906572,0.00002386307,0.00004193155,0.00209156,0.00006035483,0.001116984],"genre_scores_gemma":[0.9876916,0.0003892685,0.005551138,0.00004675349,0.00000722599,0.00005880757,0.003379039,0.0000604704,0.002815804],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.004788325,"threshold_uncertainty_score":0.009520948,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.007542032199349087,"score_gpt":0.2118921660156475,"score_spread":0.2043501338162984,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}