{"id":"W2125733997","doi":"10.1261/rna.037432.112","title":"Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags","year":2013,"lang":"en","type":"article","venue":"RNA","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Biology; RNA; CRISPR; Ribozyme; Stem-loop; T7 RNA polymerase; Guide RNA; Molecular biology; Computational biology; Trans-activating crRNA; Genetics; Cas9; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.00008578582,0.0001029076,0.0001140439,0.00002499426,0.00005331194,0.00001914622,0.00008942589,0.0001022204,0.0000389555],"category_scores_gemma":[0.0000161182,0.00008631175,0.0000341644,0.00004358774,0.00005096808,0.000005455076,0.000015974,0.0000370343,0.000002904449],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.000004338731,"about_ca_system_score_gemma":0.00003017715,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0001540936,"about_ca_topic_score_gemma":0.00001368538,"domain_scores_codex":[0.9994137,0.00004047034,0.0001291298,0.0001973724,0.00008649765,0.0001328146],"domain_scores_gemma":[0.9995686,0.00000511758,0.00007540817,0.0002271146,0.0000672227,0.00005651764],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003193961,0.00002780881,0.0001544305,0.00002091502,0.00002500328,9.795763e-7,0.00005412535,0.00001177032,0.9875194,0.0000640292,0.00004725374,0.01204235],"study_design_scores_gemma":[0.00016832,0.0001843316,0.0009514597,0.00001618911,0.00002367635,0.00001952158,0.00004238566,0.00006097919,0.9972359,0.0001672021,0.001014487,0.0001155437],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9872853,0.0008084148,0.0108782,0.00009624416,0.00003938803,0.0001897173,0.000009861439,0.000006504344,0.0006863946],"genre_scores_gemma":[0.993934,0.0001324514,0.005620928,0.00006192981,0.00004900141,0.00001873809,0.00001199526,0.00001542197,0.0001555001],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.01192681,"threshold_uncertainty_score":0.3519691,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01535619947055343,"score_gpt":0.2252305074327282,"score_spread":0.2098743079621748,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}