{"id":"W2125733997","doi":"10.1261/rna.037432.112","title":"Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags","year":2013,"lang":"en","type":"article","venue":"RNA","topic":"RNA and protein synthesis mechanisms","field":"Biochemistry, Genetics and Molecular Biology","cited_by":31,"is_retracted":false,"has_abstract":true,"ca_institutions":"Université de Montréal","funders":"Natural Sciences and Engineering Research Council of Canada; Canadian Institutes of Health Research","keywords":"Biology; RNA; CRISPR; Ribozyme; Stem-loop; T7 RNA polymerase; Guide RNA; Molecular biology; Computational biology; Trans-activating crRNA; Genetics; Cas9; Gene; Escherichia coli","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006180298,0.000670716,0.0007600684,0.000317998,0.000292919,0.00068458,0.0006115239,0.0006183933,0.001617356],"category_scores_gemma":[0.0007037431,0.0004751457,0.0008021887,0.0003324084,0.0003566526,0.0003449441,0.0006998716,0.001944299,0.002621457],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0004086136,"about_ca_system_score_gemma":0.0002590856,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004342275,"about_ca_topic_score_gemma":0.001281347,"domain_scores_codex":[0.9991258,0.0001422503,0.00007538286,0.0001701919,0.000323241,0.0001631236],"domain_scores_gemma":[0.9994424,0.0001761596,0.0001062976,0.00008976363,0.0001038112,0.00008165109],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00001614472,0.00001019747,0.00005112164,0.00003526265,0.000005876917,0.0000232543,0.0000127067,0.00009119564,0.9989222,0.0000550879,0.00004161229,0.0007354359],"study_design_scores_gemma":[0.000006085573,0.00007732372,0.0004260761,0.000005710267,0.00001196532,0.00008381068,0.0000149214,0.0008296257,0.9957628,0.00003712386,0.002736263,0.000008445645],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"methods","genre_scores_codex":[0.7687356,0.00215564,0.2174796,0.0003689652,0.0001561872,0.0004514391,0.00150065,0.001929693,0.007222232],"genre_scores_gemma":[0.8215156,0.0016907,0.1521051,0.0005889295,0.00006241885,0.0004711285,0.006900583,0.0007947223,0.01587087],"genre_candidate":"methods","genre_consensus":null,"teacher_disagreement_score":0.001617356,"threshold_uncertainty_score":0.005410552,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.01535619947055343,"score_gpt":0.2252305074327282,"score_spread":0.2098743079621748,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}