{"id":"W2127635544","doi":"10.1093/protein/gzg134","title":"The distinct heme coordination environments and heme-binding stabilities of His39Ser and His39Cys mutants of cytochrome b5","year":2003,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Hemoglobin structure and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"University of British Columbia; National Natural Science Foundation of China","keywords":"Heme; Mutant; Cytochrome b5; Cytochrome; Chemistry; Mutagenesis; Hemeprotein; Ferrous; Ferric; Ligand (biochemistry); Cytochrome b; Biochemistry; Stereochemistry; Gene; Receptor; Enzyme; Inorganic chemistry; Organic chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0002086491,0.00008668681,0.00008635878,0.00002796017,0.00006933771,0.0000104997,0.00001879341,0.00006694678,0.000002079329],"category_scores_gemma":[0.00009541516,0.00007204929,0.00001256498,0.0000488464,0.00004825419,0.00000797636,0.00001200705,0.00004381651,6.001923e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00001338962,"about_ca_system_score_gemma":0.000009870462,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00000493356,"about_ca_topic_score_gemma":0.000001264966,"domain_scores_codex":[0.9995351,0.00003747849,0.0001296698,0.0001376688,0.0000633594,0.00009666465],"domain_scores_gemma":[0.9998305,0.00001959158,0.0000523031,0.00005284727,0.00001905609,0.00002565491],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00003680056,0.000007116519,0.0009828932,0.00005098911,0.0000205898,7.808784e-8,0.00004540849,0.0001091288,0.9971884,0.0001554949,0.000005285755,0.00139783],"study_design_scores_gemma":[0.0003459721,0.0003361803,0.006639097,0.00001950366,0.00001581922,0.00001907469,0.00004164466,0.001851492,0.9890889,0.000139868,0.001397409,0.0001050384],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.8945963,0.0006215732,0.1044753,0.000008446122,0.0000256224,0.0002517155,0.000001958871,0.000004898228,0.00001418157],"genre_scores_gemma":[0.9978408,0.00009609709,0.001892204,0.000001550678,0.00001696698,0.00001803542,0.000004017586,0.000008996115,0.0001213419],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.1032444,"threshold_uncertainty_score":0.2938085,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005355110747020527,"score_gpt":0.1802910982448618,"score_spread":0.1749359874978412,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}