{"id":"W2127635544","doi":"10.1093/protein/gzg134","title":"The distinct heme coordination environments and heme-binding stabilities of His39Ser and His39Cys mutants of cytochrome b5","year":2003,"lang":"en","type":"article","venue":"Protein Engineering Design and Selection","topic":"Hemoglobin structure and function","field":"Biochemistry, Genetics and Molecular Biology","cited_by":38,"is_retracted":false,"has_abstract":true,"ca_institutions":"","funders":"University of British Columbia; National Natural Science Foundation of China","keywords":"Heme; Mutant; Cytochrome b5; Cytochrome; Chemistry; Mutagenesis; Hemeprotein; Ferrous; Ferric; Ligand (biochemistry); Cytochrome b; Biochemistry; Stereochemistry; Gene; Receptor; Enzyme; Inorganic chemistry; Organic chemistry","routes":{"ca_aff":false,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":true},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001041785,0.000247006,0.0001359447,0.0001711105,0.0001111324,0.0001481649,0.0001304398,0.0001659049,0.0004886448],"category_scores_gemma":[0.0001536199,0.0001253997,0.0001259312,0.000149267,0.0001222587,0.00008054863,0.0001297884,0.0001879481,0.0001192888],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0002027885,"about_ca_system_score_gemma":0.00009294639,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007490516,"about_ca_topic_score_gemma":0.0006751064,"domain_scores_codex":[0.9998926,0.00001780213,0.00001231393,0.00001960354,0.00003877627,0.00001891925],"domain_scores_gemma":[0.9999015,0.00001604406,0.00003305536,0.000006482893,0.00001113786,0.00003185737],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00008065235,0.00001167092,0.0003352925,0.000007520103,0.000003278023,0.00002939826,0.000009815802,0.00006256974,0.9990497,0.00003910902,0.000007316465,0.0003635914],"study_design_scores_gemma":[0.00002190941,0.0002491052,0.01271461,0.000003819901,0.00001933703,0.0004467752,0.0000304326,0.001246683,0.9845778,0.00006792689,0.0006147329,0.000006843408],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9993485,0.00008216472,0.0003270309,0.00001123996,0.000001299204,0.000002883913,0.00008893305,0.000007547848,0.0001303459],"genre_scores_gemma":[0.99876,0.00006170257,0.0005212357,0.000007712788,0.000001175021,0.000003324491,0.0003019859,0.00000922424,0.0003336701],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0007490516,"threshold_uncertainty_score":0.001634657,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.005355110747020527,"score_gpt":0.1802910982448618,"score_spread":0.1749359874978412,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}