{"id":"W2127914075","doi":"10.1007/s00253-007-0996-7","title":"Directed evolution for increased chitinase activity","year":2007,"lang":"en","type":"article","venue":"Applied Microbiology and Biotechnology","topic":"Studies on Chitinases and Chitosanases","field":"Biochemistry, Genetics and Molecular Biology","cited_by":46,"is_retracted":false,"has_abstract":false,"ca_institutions":"Brock University","funders":"","keywords":"Chitinase; DNA shuffling; Signal peptide; Biology; Directed evolution; Nucleic acid sequence; Escherichia coli; Peptide sequence; Amino acid; Biochemistry; Gene; Molecular biology; Mutant","routes":{"ca_aff":true,"ca_fund":false,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0006750489,0.001327212,0.0009522857,0.001145512,0.0002369681,0.001228218,0.001089353,0.001246149,0.002484971],"category_scores_gemma":[0.0007005599,0.0005790143,0.0006804901,0.001316323,0.0007019396,0.0003273495,0.0008908814,0.002627778,0.0006978961],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.001097517,"about_ca_system_score_gemma":0.0005142218,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0007252645,"about_ca_topic_score_gemma":0.001274147,"domain_scores_codex":[0.9994606,0.0001257791,0.00007211184,0.0000850568,0.0001575739,0.00009880604],"domain_scores_gemma":[0.9994092,0.0002132184,0.0001260692,0.00007383362,0.0000683463,0.0001093697],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001389699,0.0001292768,0.0001833425,0.0000747996,0.00003341556,0.00006984954,0.00002488468,0.000313256,0.9946103,0.0005029335,0.00006758837,0.003851437],"study_design_scores_gemma":[0.0001172506,0.0002725125,0.001344747,0.00002010147,0.0000819321,0.0003523528,0.00005144921,0.002334747,0.9921524,0.0001412488,0.003112581,0.00001860993],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9729767,0.001221021,0.01814822,0.0006713319,0.0002194735,0.0002545138,0.0008016435,0.0005105754,0.005196579],"genre_scores_gemma":[0.9783226,0.001242536,0.01448523,0.0002745616,0.00005131631,0.0001258433,0.0007466956,0.0002795606,0.004471766],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.002484971,"threshold_uncertainty_score":0.00831306,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.004460076171720027,"score_gpt":0.2175171463784533,"score_spread":0.2130570702067333,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}