{"id":"W2128888675","doi":"10.2144/000112901","title":"Design of a Single Plasmid-Based Modified Yeast One-Hybrid System for Investigation of in Vivo Protein-Protein and Protein-DNA Interactions","year":2008,"lang":"en","type":"article","venue":"BioTechniques","topic":"CRISPR and Genetic Engineering","field":"Biochemistry, Genetics and Molecular Biology","cited_by":16,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of Toronto","funders":"National Institute of General Medical Sciences; Natural Sciences and Engineering Research Council of Canada; National Institutes of Health; University of Toronto","keywords":"Fusion protein; Two-hybrid screening; Biology; Plasmid; DDB1; DNA; Molecular biology; Protein–protein interaction; In vivo; Retinoblastoma-like protein 1; Gene; Reporter gene; GATAD2B; Cell biology; Gene expression; DNA-binding protein; Biochemistry; Recombinant DNA; Genetics; Transcription factor; Repressor","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"codex-gemma-dda1882f352a","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001411391,0.0001114569,0.0001614004,0.0001029312,0.00002698919,0.000004790584,0.00007317024,0.0001042019,9.392643e-7],"category_scores_gemma":[0.00004762091,0.0001188839,0.00003949088,0.00007000944,0.00007659401,0.000004791576,0.00002446961,0.00004894266,7.149984e-8],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.00002153723,"about_ca_system_score_gemma":0.00006261503,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.00004845805,"about_ca_topic_score_gemma":0.000007984996,"domain_scores_codex":[0.9993188,0.00004157285,0.0002607383,0.0001933775,0.00007292151,0.0001125922],"domain_scores_gemma":[0.9995964,0.000008427065,0.0001122091,0.0001753963,0.00007284566,0.00003468564],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.0001273969,0.00004388238,0.00002311944,0.0003266267,0.00001062241,6.839246e-7,0.00003141945,0.0002891671,0.9989463,0.00005472892,0.00002684364,0.0001192257],"study_design_scores_gemma":[0.0003305646,0.0004819018,0.00004091277,0.0003988376,0.000006125752,0.000005461171,0.00001619684,0.002047054,0.9964065,0.00004490802,0.0001052166,0.0001163385],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.6398152,0.00006059862,0.3588986,0.00002278213,0.000008269661,0.001123961,0.00002700332,0.00002281886,0.00002081406],"genre_scores_gemma":[0.9344408,0.000004107633,0.06505381,0.000004992603,0.00002232138,0.0004067641,0.00001536925,0.00001707992,0.00003474282],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.2946257,"threshold_uncertainty_score":0.4847944,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.03021822175563036,"score_gpt":0.2690320445616162,"score_spread":0.2388138228059859,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}