{"id":"W2129538373","doi":"10.1093/nar/gkh1007","title":"Substrate specificity and kinetic framework of a DNAzyme with an expanded chemical repertoire: a putative RNaseA mimic that catalyzes RNA hydrolysis independent of a divalent metal cation","year":2004,"lang":"en","type":"article","venue":"Nucleic Acids Research","topic":"Advanced biosensing and bioanalysis techniques","field":"Biochemistry, Genetics and Molecular Biology","cited_by":60,"is_retracted":false,"has_abstract":true,"ca_institutions":"University of British Columbia","funders":"Natural Sciences and Engineering Research Council of Canada; Michael Smith Health Research BC","keywords":"Enzyme kinetics; Phosphodiester bond; Turnover number; Stereochemistry; Product inhibition; Divalent; Deoxyribozyme; Biology; Ionic strength; Substrate (aquarium); Hydrolysis; Hydrolase; Catalysis; Enzyme; Chemistry; Active site; Biochemistry; Non-competitive inhibition; DNA; RNA; Organic chemistry; Aqueous solution","routes":{"ca_aff":true,"ca_fund":true,"ca_venue":false,"about_ca":false,"invisible_to_affiliation_only":false},"retraction":null,"screen":null,"direct_labels":[],"prediction":{"model_version":"metacan-v3-hybrid-931329e0061c","candidate_categories":[],"consensus_categories":[],"category_scores_codex":[0.0001766729,0.000369784,0.0003788598,0.0001103609,0.00007224022,0.0004841732,0.000339058,0.0003888936,0.0004873345],"category_scores_gemma":[0.0002978366,0.0002137043,0.0002502936,0.00009106878,0.0002089741,0.0002718087,0.0001525893,0.0005270954,0.0003143286],"about_ca_system_candidate":false,"about_ca_system_consensus":false,"about_ca_system_score_codex":0.0005192294,"about_ca_system_score_gemma":0.0001829398,"about_ca_topic_candidate":false,"about_ca_topic_consensus":false,"about_ca_topic_score_codex":0.0004434695,"about_ca_topic_score_gemma":0.000434001,"domain_scores_codex":[0.9997827,0.00002594603,0.00001488428,0.00008247125,0.00007125131,0.00002273315],"domain_scores_gemma":[0.9998579,0.00002783456,0.00003741826,0.00001630297,0.00002681371,0.00003370778],"domain_codex":null,"domain_gemma":null,"domain_candidate":null,"domain_consensus":null,"study_design_codex":"bench_or_experimental","study_design_gemma":"bench_or_experimental","study_design_scores_codex":[0.00007097825,0.00001380161,0.0002821488,0.00002193503,0.00000536201,0.00003505204,0.000007710978,0.0006583349,0.9973845,0.00013229,0.00001145687,0.001376496],"study_design_scores_gemma":[0.000006323561,0.0001478551,0.0009183427,0.0000020326,0.000008843731,0.0002468312,0.000006263529,0.00799319,0.9898218,0.00004384342,0.0007975996,0.000007071086],"study_design_candidate":"bench_or_experimental","study_design_consensus":"bench_or_experimental","genre_codex":"empirical","genre_gemma":"empirical","genre_scores_codex":[0.9782817,0.00146804,0.01873273,0.0000630752,0.00001501386,0.00003646852,0.0001214188,0.0001791977,0.001102388],"genre_scores_gemma":[0.988493,0.0003280919,0.01003963,0.00001385251,0.000007077626,0.00002333434,0.0001418055,0.00001445313,0.0009387099],"genre_candidate":"empirical","genre_consensus":"empirical","teacher_disagreement_score":0.0005192294,"threshold_uncertainty_score":0.003767312,"prediction_status":"machine_predicted_unvalidated"},"machine_scores":{"provisional":true,"baseline":true,"maturity_gate_passed":false,"score_opus":0.02992517026910311,"score_gpt":0.3260576479887421,"score_spread":0.296132477719639,"validation_status":"score_only:v0-immature-baseline","note":"Baseline scores from an immature model (maturity gate not passed). Scores rank; they never assert a category."}}